The cytotoxic effects of DON were determined in indicated cell lines using a cell counting kit-8 (CCK-8) assay (Sangon Biotech, China). Cells were seeded in a 96-well plate at 5000 per well in the presence of the indicated concentration of DON for 24 h in a cell culture incubator. 0.5 mg/mL CCK-8 solution was added to each well for 2 h at 37 °C. The optical density of each well was determined at a wavelength of 450 nm using a microplate reader (Promega, USA).
Cell counting kit 8 cck 8 assay
Manufactured by Sangon
Sourced in China
The Cell Counting Kit-8 (CCK-8) assay is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes the highly water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in living cells to produce a colored formazan dye that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using cell counting kit 8 cck 8 assay
1
Cytotoxicity Evaluation of DON
2
Cell Viability Determination using CCK-8 Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell counting kit-8 (CCK-8) assay (Sangon Biotech, China) was applied to determine the cell viability of HK-2 cells. Cells were cultured in a 96-well plate at a density of 5,000 cells per well. 0.5 mg/mL CCK-8 (10 μL) solution was further added to each well, and cells were incubated for another 1 h at 37°C. The optical density was characterized at 450 nm by a microplate reader (Promega, USA).
3
Cytotoxicity Evaluation of Biomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cytotoxicity tests were conducted according to ISO10993-5-2009. Extracts were obtained by rinsing samples with PBS solution three times followed by impregnation with Dulbecco's modified Eagle's medium (DMEM; Gibco, USA) for 24 h, the extraction condition of samples was set as 6 cm2/mL, 37 °C. DMEM was mixed with 5% dimethyl sulfoxide and pure DMEM solutions were set as the positive and negative control groups. L929 cells (The Cell Bank of Type Culture Collection of Chinese Academy of Science, Shanghai, China) were seeded in a 96-well plate at a density of 3000 cells/mL and placed in a 5% CO2 incubator at 37 °C for 24 h. Solutions corresponding to the groups (100 μL) were added to each well and the medium was changed every 24 h. On days one and three, the morphology of cells was examined by microscopy, and the viability of L929 was determined by the Cell Counting Kit-8 (CCK-8) assay (Sangon Biotech Co., Ltd., Shanghai, China).
4
Cell Proliferation Assay using CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation was measured using a Cell Counting Kit-8 (CCK-8) assay (Sangon Biotech, Co., Ltd., Shanghai, China). A total of 48 h post-transfection, 3×103 cells/well were seeded into a 96-well plate. The CCK-8 reagent was added 72 h post-seeding, and optical density was measured using a microplate reader at 450 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!