Cd28 bv711

Manufactured by BioLegend

Sourced in United States

CD28-BV711 is a fluorophore-conjugated antibody that binds to the CD28 protein. CD28 is a co-stimulatory receptor expressed on the surface of T cells and plays a crucial role in T cell activation and proliferation.

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Lab products found in correlation

Cd38 bv421, by BioLegend (2 mentions) Tigit pe cy7, by Thermo Fisher Scientific (2 mentions) Cd45ra af700, by BD (2 mentions) Cd160 af488, by BD (2 mentions) Tim 3 bv650, by BD (2 mentions) Cd27 bv650, by BioLegend (2 mentions) Lag 3 apc, by Thermo Fisher Scientific (2 mentions) Hla dr af700, by BioLegend (2 mentions) Ccr7 bv421, by BioLegend (2 mentions) Pd 1 bv711, by BD (2 mentions) Lsrfortessa flow cytometer, by BD (2 mentions) 2b4 fitc, by BD (1 mentions) Cd8 bv510, by BD (1 mentions) Anti human cd3 bv786, by BD (1 mentions) Cd70 pe, by BD (1 mentions) Cd4 apc fire750, by BD (1 mentions) Cd95 pecy7, by BD (1 mentions) Cd8 fitc, by BioLegend (1 mentions) Cd38 bv510, by BioLegend (1 mentions) Cd57 apc, by BioLegend (1 mentions)

4 protocols using cd28 bv711

Multiparametric Flow Cytometry of PBMCs

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PBMCs were incubated with directly conjugated antibodies for 30 min at 4°C. The cells were washed before flow cytometry analysis. Antibodies used included anti-human CD3-BV786, CD4-APC-Fire750, CD8-BV510, CD45RA-AF700, CD70-PE, PD-1-BV711, 2B4-FITC, CD160-AF488, TIM-3-BV650, CD95-PE-CY7 (BD Biosciences, San Diego, CA, USA), CCR7-BV421, HLA-DR-AF700, CD38-BV421, CD28-BV711, CD27-BV650 (BioLegend, San Diego, CA, USA), TIGIT-PE-Cy7, LAG-3-APC (Ebioscience, San Diego, CA, USA) and the corresponding isotype controls. Data acquisition was performed on an LSR Fortessa flow cytometer (BD Biosciences), and data was analyzed with FlowJo software (Tree Star, Ashland, OR, USA).

Wang D., Du J., Song Y., Wang B., Song R., Hao Y., Zeng Y., Xiao J., Zheng H., Zeng H., Zhao H, & Kong Y. (2020). CD70 contributes to age-associated T cell defects and overwhelming inflammatory responses. Aging (Albany NY), 12(12), 12032-12050.

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Comprehensive Immunophenotyping of PBMC

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To immunophenotype cell populations, PBMC were thawed, washed, split into two panels, and stained for 10 min in the dark at room temperature with antibodies against the following human surface markers: CD3 Percp.Cy5.5 (BD-Biosciences, San Jose, CA, USA), CD4, CD8-FITC, CD45RO-APC, CD197-AlexaFluor-700, CD38-BV510, HLA-DR-PE.Cy7, CD28-BV711, CD27 PE-Dazzle-594, CD57-APC, and CD107a BV421(Biolegend, San Diego, CA, USA). The cells were fixed using Perm/fix (BD-Biosciences, San Jose, CA, USA) for 20 min at 4 °C in the dark, and then stained for intracellular Ki67 (Biolegend, San Diego, CA, USA) or IFN-γ (Biolegend, San Diego, CA, USA). Raw data were quantified by FACSAria (BD-Biosciences, San Jose, CA, USA) and analyzed with FlowJo version-10.

Lidenge S.J., Tso F.Y., Mortazavi Y., Ngowi J.R., Shea D.M., Mwaiselage J., Wood C, & West J.T. (2020). Viral and Immunological Analytes are Poor Predictors of the Clinical Treatment Response in Kaposi’s Sarcoma Patients. Cancers, 12(6), 1594.

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Phenotypic Profiling of Cryopreserved PBMCs

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Cryopreserved PBMCs were thawed, as described above. A total of 1 × 10⁶ live PBMCs were labeled with peptide-MHC class I Pentamer-PE (ProImmune) and incubated for 15 min at 37 °C. Dead cells were first labeled with LIVE/DEAD Fixable Aqua dye (Invitrogen) and then with the surface markers CD3-BUV395, CD8-PerCP.Cy5.5, CD14-BV510, CD16-BV510, CD19-BV510, CD28-BV711, CD27-APC-R700, CD45RA-APC-H7 and CCR7-PE-Dazzel 594 (BioLegend). All reagents were from BD Biosciences unless otherwise stated. All samples were acquired on a BD LSRFortessa (BD Biosciences) flow cytometer and analyzed using FlowJo version 10 software.

Peng Y., Mentzer A.J., Liu G., Yao X., Yin Z., Dong D., Dejnirattisai W., Rostron T., Supasa P., Liu C., López-Camacho C., Slon-campos J., Zhao Y., Stuart D.I., Paesen G.C., Grimes J.M., Antson A.A., Bayfield O.W., Hawkins D.E., De-Sheng K., Wang B., Turtle L., Subramaniam K., Thomson P., Zhang P., Dold C., Ratcliff J., Simmonds P., de Silva T., Sopp P., Wellington D., Rajapaksa U., Chen Y.L., Salio M., Napolitani G., Paes W., Borrow P., Kessler B.M., Fry J.W., Schwabe N.F., Semple M.G., Baillie J.K., Moore S.C., Openshaw P.J., Ansari M.A., Dunachie S., Barnes E., Frater J., Kerr G., Goulder P., Lockett T., Levin R., Zhang Y., Jing R., Ho L.P., Cornall R.J., Conlon C.P., Klenerman P., Screaton G.R., Mongkolsapaya J., McMichael A., Knight J.C., Ogg G, & Dong T. (2020). Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19. Nature immunology, 21(11), 1336-1345.

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Comprehensive Immune Phenotyping of PBMCs

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PBMCs from healthy subjects were resuspended in PBS buffer and were incubated with directly conjugated antibodies for 30 min at 4°C. The cells were washed with 1× PBS before flow cytometry analysis. Antibodies used included anti-human CD3-BV786 or CD3-BUV737, CD8-BV510 or CD8-BUV395, CD160-AF488, CD45RA-AF700, CD28-APC, CD95-PE, CD57-BV421, PD-1-BV711, TIM-3-BV650 (BD Biosciences, San Diego, CA, USA), CD4-APC-Fire750, CD244-PE-D594, CCR7-BV421, HLA-DR-AF700, CD38-BV421, CD28-BV711, CD27-BV650, KLRG-1-APC-Fire750, CD95-PE-CY7 (BioLegend, San Diego, CA, USA), TIGIT-PE-Cy7, LAG-3-APC (Ebioscience, San Diego, CA, USA) and the corresponding isotype controls. Data were acquired with the LSR Fortessa flow cytometer (BD Biosciences) and analyzed with FlowJo software version 10.5 (Tree Star, Ashland, OR, USA). More information about antibodies is listed in the Supplementary Material (Table S2).

Wang X., Wang D., Du J., Wei Y., Song R., Wang B., Qiu S., Li B., Zhang L., Zeng Y., Zhao H, & Kong Y. (2022). High Levels of CD244 Rather Than CD160 Associate With CD8+ T-Cell Aging. Frontiers in Immunology, 13, 853522.

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