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Cellsqiaamp dna mini kit

Manufactured by Qiagen
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Sourced in Germany

The CellsQIAamp® DNA Mini Kit is a laboratory product designed for the purification of genomic DNA from various cell types. It utilizes a silica-based membrane technology to efficiently capture and purify DNA, making it suitable for a range of downstream molecular biology applications.

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Lab products found in correlation

Ampflstr identifier plus pcr amplification kit, by Thermo Fisher Scientific (2 mentions) 3730xl genetic analyzer, by Thermo Fisher Scientific (2 mentions) Atcc crl 3271, by American Type Culture Collection (1 mentions)

2 protocols using cellsqiaamp dna mini kit

1

Genomic Fingerprinting of HTR-8/SVneo Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was extracted from HTR-8/SVneo cellsQIAamp® DNA Mini Kit (Qiagen; Hilden, Germany). Microsatellite genotyping was performed using AmpFLSTR Identifier Plus PCR Amplification Kit run on a 3730XL Genetic Analyzer (Applied Biosystems; Waltham, MA, USA). The short tandem repeat profile generated for our cells was compared to the short tandem repeat profile for HTR-8/SVneo (ATCC® CRL-3271™) published by American Type Culture Collection (Manassas, VA, USA).51 The short tandem repeat profile was a match: CSF1PO: 12, D13S317: 9,12, D16S539: 13D5S818: 12, D7S820: 12, TH01: 6,9.3, vWA: 13,18, TPOX: 8, Amelogenin gender determination marker: X.51
Elkin E.R., Bridges D., Harris S.M, & Loch-Caruso R.K. (2020). Exposure to Trichloroethylene Metabolite S-(1,2-Dichlorovinyl)-L-cysteine Causes Compensatory Changes to Macronutrient Utilization and Energy Metabolism in Placental HTR-8/SVneo Cells. Chemical Research in Toxicology, 33(6), 1339-1355.
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2

Genomic DNA Profiling of HTR-8/SVneo Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA
was extracted from
HTR-8/SVneo cellsQIAamp DNA Mini Kit (Qiagen; Hilden, Germany). Microsatellite
genotyping was performed using AmpFLSTR Identifier Plus PCR Amplification
Kit run on a 3730XL Genetic Analyzer (Applied Biosystems; Waltham,
MA, USA). The short tandem repeat profile generated for our cells
was compared to the short tandem repeat profile for HTR-8/SVneo (ATCC
CRL-3271) published by American Type Culture Collection (Manassas,
VA, USA).51 The short tandem repeat profile
was a match: CSF1PO, 12; D13S317, 9, 12; D16S539, 13; D5S818, 12;
D7S820, 12; TH01, 6, 9.3; vWA, 13, 18; TPOX, 8; amelogenin gender
determination marker, X.51
Elkin E.R., Bridges D., Harris S.M, & Loch-Caruso R.K. (2020). Exposure to Trichloroethylene Metabolite S-(1,2-Dichlorovinyl)-L-cysteine Causes Compensatory Changes to Macronutrient Utilization and Energy Metabolism in Placental HTR-8/SVneo Cells. Chemical Research in Toxicology, 33(6), 1339-1355.
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