Electrochemical experiments were performed on a Gamry Potentiostat Interface 1000 controlled with software PHE 200 and PV 220. All measurements were carried out in a single-compartment electrochemical cell with a standard three-electrode arrangement. A platinum disk with an area of 0.72 cm2 and a platinum wire were used as the working and the counter electrodes, respectively. The working electrode was successively polished with 1.0, 0.3 and 0.05 μm alumina slurries (Buehler) on microcloth pads (Buehler). After each polishing, the electrode was washed with water and sonicated for 10 min in acetonitrile. Then, it was immersed into a hot piranha solution (3:1, H2SO4, 30% H2O2) for 10 min, and rinsed copiously with water. All potentials were reported versus Ag/AgCl/KCl (3.0 M) reference electrode (BAS Model MF-2078) at room temperature. The electrolyte solutions were degassed with purified nitrogen for 10 min before each experiment and bubbled with nitrogen during the experiment. Operating conditions for SWV were pulse amplitude 25 mV, frequency 10 Hz, potential step 4 mV; and for DPV were pulse amplitude 50 mV, pulse width 50 ms, scan rate 100 mV/s.
Interface 1000 potentiostat
Manufactured by Gamry
Sourced in United States
The Interface 1000 potentiostat is a laboratory instrument designed for electrochemical measurements. It provides precise control and measurement of electrochemical cells. The device can apply potential and measure current, enabling researchers to study a variety of electrochemical processes and materials.
Automatically generated - may contain errors
Lab products found in correlation
10 protocols using interface 1000 potentiostat
1
Electrochemical Characterization of Platinum Electrode
2
Cyclic Voltammetry of Nitrophenoxy Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cyclic voltammetric analysis was performed using Gamry Potentiostat Interface 1000 with glassy carbon (GC) working electrode (active area of 0.07 cm2) against Ag/AgCl reference with Pt wire as counter electrode.
The solvent background scan was run in the selected potential range of bisnitrophenoxy compounds. All CV profiles were recorded in the inert environment created by purging argon gas to avoid exposure of O2. In order to compensate the IR/ohmic drop and to uphold the vicinity of working and reference electrode, three-electrode cell configuration was employed. Prior to each measurement, GC was polished and cleaned using fine alumina, later it was thoroughly washed with distilled water followed by the working solvent. As the dsDNA is electrochemically inactive in the potential window of GC, therefore the solutions of these compounds were CV titrated against dsDNA.
The solvent background scan was run in the selected potential range of bisnitrophenoxy compounds. All CV profiles were recorded in the inert environment created by purging argon gas to avoid exposure of O2. In order to compensate the IR/ohmic drop and to uphold the vicinity of working and reference electrode, three-electrode cell configuration was employed. Prior to each measurement, GC was polished and cleaned using fine alumina, later it was thoroughly washed with distilled water followed by the working solvent. As the dsDNA is electrochemically inactive in the potential window of GC, therefore the solutions of these compounds were CV titrated against dsDNA.
3
Fabrication and Application of HOCl-Producing e-Bandages
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HOCl‐producing e‐bandages were constructed as previously described (Mohamed et al., 2021 (link)), and autoclaved for 15 min at 121°C before use against biofilms. e‐Bandages were polarized at 1.5VAg/AgCl to generate HOCl. e‐Bandages are a three‐electrode system consisting of a conductive carbon fabric (Panex 30 PW‐06, Zoltek) as working and counter electrode, and an Ag/AgCl reference wire as a reference electrode. e‐Bandages were applied with the working electrode facing the biofilm. A Gamry Interface1000 potentiostat and Gamry ECM8 were used to control working electrode potential (Gamry Instruments).
4
Nitinol Wire Fatigue Behavior in PBS
5
Potentiostat Characterization of Semiconductors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EIS were measured
by a potentiostat (Interface 1000 potentiostat; Gamry Instruments)
with a three-electrode system in a 0.5 M Na2SO4 solution (pH 6.5). The potential was measured against an Ag/AgCl
reference and converted to NHE potentials by using E(NHE) = E(Ag/AgCl) + 0.197 V. In the case of the
Mott–Schottky analysis, it can be estimated from the intersection
of a plot of 1/C2 against E by the following equation where C is the space charge
capacitance (F cm–2), e is the
elementary charge (1.62 × 10–19 C), ϵ
the relative dielectric constant of the semiconductor, ϵ0 is the permittivity of vacuum (8.85 × 10–12 N–1 C2 m–2), N is an acceptor density, E is the applied
potential (V), k is the Boltzmann constant (1.38
× 10–23 J K–1), and T is the absolute temperature (K).
by a potentiostat (Interface 1000 potentiostat; Gamry Instruments)
with a three-electrode system in a 0.5 M Na2SO4 solution (pH 6.5). The potential was measured against an Ag/AgCl
reference and converted to NHE potentials by using E(NHE) = E(Ag/AgCl) + 0.197 V. In the case of the
Mott–Schottky analysis, it can be estimated from the intersection
of a plot of 1/C2 against E by the following equation where C is the space charge
capacitance (F cm–2), e is the
elementary charge (1.62 × 10–19 C), ϵ
the relative dielectric constant of the semiconductor, ϵ0 is the permittivity of vacuum (8.85 × 10–12 N–1 C2 m–2), N is an acceptor density, E is the applied
potential (V), k is the Boltzmann constant (1.38
× 10–23 J K–1), and T is the absolute temperature (K).
6
Pitting Corrosion Susceptibility Evaluation
7
Anodic Spike Voltammetry of Iron Oxide NPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anodic Spike Voltammetry (ASV) was conducted using the microfabricated three electrode system connected to Interface 1000 potentiostat (Gamry Instruments). NPs concentration were adjusted to be 100 µM. The three electrode system consisted of microfabricated Pt working electrode, Pt counter electrode and a Ag/AgCl reference electrode. Using the known standard reduction potential of the reference electrode, the potential applied to the working electrode by means of the potentiostat was controlled and regulated and the redox reactions were studied by measurement of the current. In this experiment, the Pt working electrode was held at a constant potential of -1.5 V for 120 s and then scanned towards 1.5 V vs. the Ag/AgCl reference electrode53 . The other important parameters for this experiment includes a frequency of 25 Hz and a pulse size of 25 mV.
The iron oxide NPs are accumulated around the reference electrode when held at a constant potential while gaining electrons. Subsequently, when the potential is scanned towards 1.5 V, the iron oxide NPs return into the solution and the measured current corresponds to their concentration on the surface.
The iron oxide NPs are accumulated around the reference electrode when held at a constant potential while gaining electrons. Subsequently, when the potential is scanned towards 1.5 V, the iron oxide NPs return into the solution and the measured current corresponds to their concentration on the surface.
8
Determining HiPIP-41 Redox Potential
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The redox potential of HiPIP-41 was determined directly via classic cyclic voltammetry (CV). Control potential scans of the buffer without HiPIP-41 showed redox peaks. HiPIP-41 (600 μM) was applied directly as thin protein film on the freshly polished surface (0.07 cm2) of a glassy carbon working electrode. The working electrode was inserted into a Slide-A-Lyzer Mini Dialysis Unit (MWCO 3.5 kDa; Pierce) in order to prevent protein dilution during CV measurements. The dialysis unit was inserted into a buffer filled glass vessel together with the platinum counter electrode and the Ag/AgCl (in 3 M NaCl) reference electrode. CV experiments were conducted using a Gamry Interface1000 potentiostat and the Gamry Framework software. Data was collected between potential limits of 200 and 900 mV vs. SHE with potential scan rates of 10, 50 and 100 mV/s which were applied in direct succession to the assay. The Em of HiPIP-41 was determined based on the potentials of its oxidative and reductive peaks. A correction factor of 197 mV was used to convert redox potentials from vs. Ag/AgCl (3 M NaCl) to vs. standard hydrogen electrode (SHE).
9
Quantifying Hydrogen Peroxide in Hydroponic Media
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Horseradish peroxidase (HRP) was immobilized on DRP-C110 screen-printed carbon electrodes (DropSens, Metrohm, Australia) following previously described methods (27 (link), 41 (link)–43 (link)) and continuously calibrated against fresh standard H2O2 solutions. H2O2 concentrations in hydroponic media from the growth pouches for the different treatments were assessed by aspirating 1 mL of growth medium from the growth pouches into a 2-mL cuvette. An electrode (prepared as defined above) was submerged into the 2-mL cuvette containing 1 mL of medium and H2O2 levels were quantified based on the voltage through the sensor, which was measured using a Gamry Interface 1000 potentiostat (Gamry, Warminster, PA, USA) as previously described (27 (link)).
10
Electrochemical Glucose Sensing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All electrochemical experiments including cyclic voltammetry (CV) and chronoamperometry (CA) were performed by a Gamry Instruments Interface 1000 Potentiostat/Galvanostat in a standard three-electrode electrochemical cell configuration. Electrodeposited PB on FTO substrate is used as a working electrode with an area of 1 ± 0.05 cm 2 , a Pt wire serves as a counter electrode, and Ag/AgCl (3 M KCl) is used as a reference electrode. All of the calculations are based on the geometric surface area and all potentials measured vs. Ag|AgCl|KCl(sat.). CV is performed at a scan rate of 50 mV s À1 with a potential range from 0.2 to 1.3 V Ag/AgCl , while a potential of 1.15 V Ag/AgCl is applied for amperometric experiments in a N 2 -saturated 0.1 M phosphate buffer solution (PBS).
For the amperometric detection of glucose, the current response is recorded while a specific concentration of glucose (from 0 to 10 mM) is added to a solution of 0.1 M PBS containing 0.1 M KCl at a specific potential. Each steady-state current response is recorded to obtain a steady-state current/glucose concentration profile to evaluate the sensing ability of the electrodes. Electrochemical experiments are performed also with the addition of interfering agents including fructose, sucrose, uric acid, and ascorbic acid.
For the amperometric detection of glucose, the current response is recorded while a specific concentration of glucose (from 0 to 10 mM) is added to a solution of 0.1 M PBS containing 0.1 M KCl at a specific potential. Each steady-state current response is recorded to obtain a steady-state current/glucose concentration profile to evaluate the sensing ability of the electrodes. Electrochemical experiments are performed also with the addition of interfering agents including fructose, sucrose, uric acid, and ascorbic acid.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!