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Glimepiride

Manufactured by Cayman Chemical
Sourced in United States

Glimepiride is a laboratory reagent used for scientific research purposes. It is a synthetic sulfonylurea compound. Glimepiride functions as an oral antidiabetic agent that stimulates the release of insulin from the pancreas. Its core function is to serve as a tool for investigating diabetes-related mechanisms and processes in a laboratory setting.

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2 protocols using glimepiride

1

Fluorescent Glucose Uptake Analysis

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β-Cyclodextrin was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and 20(S)-protonaxadiol 20-O-D-glucopyranoside (compound K) was obtained from Climax Biotech Co., Ltd. (Chengdu, China). Alloxan monohydrate and sea salts were obtained from Sigma Chemical Co. (St. Louis, MO, USA), and 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) was purchased from Invitrogen Co., Ltd. (Grand Island, NY, USA). Glimepiride and diazoxide were purchased from Cayman Chemical Co. (Ann Arbor, MI, USA) and Santa Cruz Biotechnology Inc. (Dallas, TX, USA), respectively. Fluorescence microscopy was conducted using an Olympus 1X70 microscope (Olympus Co., Ltd., Tokyo, Japan). Focus Lite (Focus Co, Daejeon, Korea) and Image J (National Institutes of Health, Bethesda, MD, USA) software were used for image analysis.
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2

Recombinant Insulin Glucose Uptake

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Insulin Human Recombinant, D-glucose, Pioglitazone, ile-pro-ile, acarbose tricaine methanesulfonate and sea salts were purchased from Sigma Chemical Co. (St. Louis, MO, USA). 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) was purchased from Invitrogen (Carlsbad, CA, USA). Glimepiride was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA). Fluorescence microscopy was performed using an Olympus 1 × 70 microscope (Tokyo, Japan). For image analysis, Focus Lite (Focus Co, Gwangmyeong-si, Korea) and Image J (V 1.50i, National Institutes of Health, Bethesda, MD, USA) were used.
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