Disodium inosine 5 monophosphate

Nucleotide standard: disodium guanosine 5′-monophosphate (5′-GMP, with purity 99.0%), disodium cytidine 5′-monophosphate (5′-CMP, with purity 99.0%), disodium uridine 5′-monophosphate (5′-UMP, with purity 99.0%), disodium inosine 5′-monophosphate (5′-Imp, with purity 99.0%), disodium adenosine 5′-monophosphate (5′-AMP, with purity 99.0%), and disodium xanthin 5′-monophosphate (5′-XMP, with purity 98.0%) were bought from Sigma company; 16 kinds of standard amino acids, methanol, acetonitrile (special for chromatography) were purchased from Thermo Fisher Technology Co., Ltd., Waltham, MA, USA; concentrated hydrochloric acid (HCl), trichloroacetic acid, sodium hydroxide, sulfosalicylic acid, potassium dihydrogen phosphate, arc bis (polyacrylamide), 10% APS (ammonium persulfate), TEMED (tetramethylethylenediamine), Tris base (trimethylolaminomethane), glycine, SDS (sodium dodecyl sulfate), 1m Tris HCl (trimethylolaminomethane hydrochloride, pH 6.8), glycerol, 2-mercaptoethanol, bromophenol blue, Brilliant Blue R and other chemicals were of analytical grade and bought from Chengdu Cologne Chemicals Co., Ltd., Chengdu, China.