Rabbit anti jnk

Manufactured by Abcam

Sourced in United States

Rabbit anti-JNK is a primary antibody that specifically binds to the c-Jun N-terminal kinase (JNK) protein. JNK is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in cellular processes such as gene expression, cell proliferation, and apoptosis. This antibody can be used for the detection and quantification of JNK in various experimental applications.

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Close spelling variants of this product

Rabbit anti-phospho-JNK Rabbit anti-p-JNK Rabbit JNK Anti-JNK Rabbit anti-

3 protocols using rabbit anti jnk

Protein Expression Analysis in Cells

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To determine APN, LC3B, JNK/P-JNK, Akt/P-Akt, P62, and cleaved-caspase-3/pro-caspase-3 levels, proteins were extracted from the cells by suspension in radioimmunoprecipitation assay buffer. Samples were centrifuged at 12,000 rpm at 4°C for 30 min, and the supernatants were recovered for analysis. The protein concentrations were determined using the Bradford protein method and the bicinchoninic acid protein assay kit (Sigma, St Louis, MO, USA). Protein (40 µg) was electrophoresed on a pre-cast bis-Tris polyacrylamide gel (8%~12%) and then transferred onto a polyvinylidene difluoride membrane. Membranes were blotted with rabbit anti-APN (1:1,000), rabbit anti-LC3B (1:500), rabbit anti-JNK (1:1,000), rabbit anti-p-JNK (1:1,000), rabbit anti-Akt (1:1,000), rabbit anti-p-Akt (1:1,000), mouse anti-P62 (1:1,000), rabbit anti-Caspase-3 (1:1,000) (all from Abcam, San Francisco, CA, USA), and mouse anti-actin (1:1,000; Proteintech, NY, USA), followed by horseradish peroxidase-conjugated secondary antibodies (1:5,000; ZsBio, Beijing, China). Immunoblots were visualized using enhanced chemiluminescence (LAS-4000).

Wang X., Liu Y., Wu R., Guo F., Zhang L., Cui M., Wu X., Zhang Y, & Liu W. (2018). Role of ubenimex as an anticancer drug and its synergistic effect with Akt inhibitor in human A375 and A2058 cells. OncoTargets and therapy, 11, 943-953.

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Investigating Cytoskeletal Regulation in Muscle

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The primary antibodies used in the study included rabbit anti-Profilin1 (1:1000, Abcam, ab50667), rabbit anti-Cdc42 (1:1000, Proteintech, Rosemont, IL, USA), rabbit anti-PAK (1:1000, Proteintech, Rosemont, IL, USA), rabbit anti-Phospho PAK (1:1500, Proteintech, IL, USA), rabbit anti-IgG (1:50, Santa Cruz, Dallas, TX, USA), rabbit anti-JNK (1:1000, Abcam, ab179461, London, UK), rabbit anti-Phospho JNK(1:1000, Abcam, ab124956, London, UK), mouse anti-MyHC (1:100, DSHB, CO, USA), mouse anti-MyoG (1:100, DSHB, Iowa, IA, USA), mouse anti-GAPDH (1:1000, Zhongshan, Golden Bridge Bio-technology, Beijing, China), and mouse anti-tubulin (1:3000, Proteintech, Rosemont, IL, USA). The secondary antibodies included horseradish peroxidase goat anti-mouse/rabbit IgG (1:10000, Zhongshan, Golden Bridge Bio-technology, Beijing, China), PAK inhibitors (Selleck, PF-3758309), and JNK inhibitors (GLPBIO, SP600125). A G-LISA^® Cdc42 Activation Assay Biochem Kit™ (cytoskeleton, Cat. # BK127-S) was used.

Zi J., Xu J., Luo J., Yang X., Zhen Z., Li X., Hu D., Guo Y., Guo H., Ding X, & Zhang L. (2022). PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK. Cells, 11(20), 3188.

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Western Blot Analysis of Signaling Pathways

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After extracting the protein with RIPA containing 1% protease inhibitor and phosphatase inhibitor (Beyotime, China), the sample lysates were subjected to SDS–PAGE and the proteins on the gel were transferred to PVDF membranes. The membranes were incubated with the following primary antibodies at 4 °C for 12 h: rabbit anti-p65 (Cell Signaling Technology, 1:1 000, USA), rabbit anti-p-p65 (Cell Signaling Technology, 1:1 000, USA), rabbit anti-p38 (Cell Signaling Technology, 1:1 000, USA), rabbit anti-p-p38 (Cell Signaling Technology, 1:1 000, USA), rabbit anti-ERK (Cell Signaling Technology, 1:1 000, USA), rabbit anti-p-ERK (Cell Signaling Technology, 1:1 000, USA), rabbit anti-JNK (Abcam, 1:1 000, UK), rabbit anti-p-JNK (Cell Signaling Technology, 1:1 000, USA), and rabbit anti-GAPDH (Cell Signaling Technology, 1:1 000, USA). HRP-conjugated goat anti-rabbit secondary antibody (Signalway Antibody, 1:5 000, USA) was combined with the primary antibody and allowed to react with the substrate. The pixel densities of the protein bands were measured with ImageJ software (v1.8.0, USA).

Sun L., He L., Wu W., Luo L., Han M., Liu Y., Shi S., Zhong K., Yang J, & Li J. (2021). Fibroblast membrane-camouflaged nanoparticles for inflammation treatment in the early stage. International Journal of Oral Science, 13, 39.

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