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Irf1 mice

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Irf1–/– mice are genetically modified mice with a targeted deletion of the Irf1 gene, which encodes the transcription factor interferon regulatory factor 1 (IRF1). These mice can be used as a model to study the biological functions of IRF1 and its role in various cellular processes.

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C57bl 6j, by Jackson ImmunoResearch (1 mentions) C57bl 6j bl6, by Jackson ImmunoResearch (1 mentions)

5 protocols using irf1 mice

1

Genetically Modified Mice for Research

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Irf1–/– mice were obtained from Jackson Laboratory (Bar Harbor, ME), Sphk1–/– mice were provided by Dr. Richard Proia, National Institutes of Health (Bethesda, MD), and Stat1–/– mice were provided by Dr. Andrew Larner, VCU (Richmond, VA). Irf3–/–Irf7–/– double-knockout mice46 (link) were generated and housed at WUSM, while all other mice were housed at VCU according to guidelines of VCU Institutional Animal Care and Use Committee and mouse protocols were approved by the institutional IACUC. 8-16 week old male and female mice were used.
Harikumar K.B., Yester J.W., Surace M.J., Oyeniran C., Price M.M., Huang W.C., Hait N.C., Allegood J.C., Yamada A., Kong X., Lazear H.M., Bhardwaj R., Takabe K., Diamond M.S., Luo C., Milstien S., Spiegel S, & Kordula T. (2014). K63-linked polyubiquitylation of IRF1 transcription factor is essential for IL-1-induced CCL5 and CXCL10 chemokine production. Nature immunology, 15(3), 231-238.
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2

AOM-DSS Colorectal Cancer and Colitis Models

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For the CA-CRC model19 (link), 8–10 week-old C57BL/6J, A/J, or Irf1−/− mice (The Jackson Laboratory) were injected ip with azoxymethane (AOM; 7 mg/kg) followed by three 4-day cycles of 2% (w/v) DSS in the drinking water, with each cycle 17 days apart and euthanized 6 weeks later. In some experiments, mice were injected with AOM (7 mg/kg) once, followed by two 4-days cycles of 2% (w/v) DSS and sacrificed 3 weeks later. The model of AOM-induced colorectal cancer was as previously described20 (link). For the chronic colitis model, mice were treated with three 4-days cycles of 2% (w/v) DSS and sacrificed after week 8.
Jeyakumar T., Fodil N., Van Der Kraak L., Meunier C., Cayrol R., McGregor K., Langlais D., Greenwood C.M., Beauchemin N, & Gros P. (2019). Inactivation of Interferon Regulatory Factor 1 Causes Susceptibility to Colitis-Associated Colorectal Cancer. Scientific Reports, 9, 18897.
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3

Harvesting Organs from Genetically-Modified Mice

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C57BL/6J (BL6) and IRF-1−/− mice (Matsuyama et al., 1993 (link)) were obtained from The Jackson Laboratory (Bar Harbor, Maine). IFNAR1−/− mice on the BL6 background were a gift from Dr. Mitchell Grayson (Grayson et al., 2007 (link)). IRF-3−/− and IRF-7−/− mice were a gift from Dr. Michael Diamond (Honda et al., 2005 (link); Sato et al., 2000 (link)). All mice utilized in this study are on the C57BL/6J background. Organs were harvested from naïve mice between 8 and 12 weeks of age. Mice were housed and bred in a specific-pathogen-free barrier facility in accordance with federal and institutional guidelines. All experimental manipulations of mice were approved by the Institutional Animal Care and Use Committee of the Medical College of Wisconsin.
Stoltz K.P., Jondle C.N., Pulakanti K., Sylvester P., Urrutia R., Rao S, & Tarakanova V.L. (2018). Tumor suppressor Interferon Regulatory Factor 1 selectively blocks expression of endogenous retrovirus.. Virology, 526, 52-60.
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4

CKD Model and IS Treatment in Mice

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IRF1 knockout (IRF1-/-) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). To construct a CKD model, 8-week-old male Balb/c mice received electrocoagulation of right 2/3 renal cortex first and left total nephrectomy two weeks later, as previously described 16 (link). CKD mice were fed a diet containing 5% AST-120 (Kremezin, Kure ha Chemical Industry, Tokyo, Japan) for 8 weeks. WT C57BL/6J and IRF1-/- mice were intraperitoneally injected with IS (100 mg/kg, Sigma-Aldrich, St. Louis, MO, USA) daily for 8 weeks. Each group included at least 8 mice. All animal procedures were approved by the Committee of Ethics on Animal Experiments of the Army Medical University.
Huang Y., Zhou J., Wang S., Xiong J., Chen Y., Liu Y., Xiao T., Li Y., He T., Li Y., Bi X., Yang K., Han W., Qiao Y., Yu Y, & Zhao J. (2020). Indoxyl sulfate induces intestinal barrier injury through IRF1-DRP1 axis-mediated mitophagy impairment. Theranostics, 10(16), 7384-7400.
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5

Genetically Modified Mice for Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Irf1–/– mice were obtained from Jackson Laboratory (Bar Harbor, ME), Sphk1–/– mice were provided by Dr. Richard Proia, National Institutes of Health (Bethesda, MD), and Stat1–/– mice were provided by Dr. Andrew Larner, VCU (Richmond, VA). Irf3–/–Irf7–/– double-knockout mice46 (link) were generated and housed at WUSM, while all other mice were housed at VCU according to guidelines of VCU Institutional Animal Care and Use Committee and mouse protocols were approved by the institutional IACUC. 8-16 week old male and female mice were used.
Harikumar K.B., Yester J.W., Surace M.J., Oyeniran C., Price M.M., Huang W.C., Hait N.C., Allegood J.C., Yamada A., Kong X., Lazear H.M., Bhardwaj R., Takabe K., Diamond M.S., Luo C., Milstien S., Spiegel S, & Kordula T. (2014). K63-linked polyubiquitylation of IRF1 transcription factor is essential for IL-1-induced CCL5 and CXCL10 chemokine production. Nature immunology, 15(3), 231-238.
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