Acetic acid (99.5%), aluminum chloride hexahydrate, copper(II) chloride dihydrate, disodium hydrogen phosphate dihydrate, 96% ethanol, 36% hydrochloric acid, iron(III) chloride hexahydrate, methanol, phosphoric acid, potassium persulfate, potassium dihydrogen phosphate, potassium hexacyanoferrate(III), sodium acetate anhydrous, sodium carbonate anhydrous, sodium hydroxide, sodium nitrite, and trichloroAcetic acid were purchased from Chempur, Poland. Neocuproine was delivered by J&K Scientific, Germany. Folin–Ciocalteu reagent, acetonitrile, iron(II) sulfate heptahydrate, gallic acid were supplied by Merck, Germany, whereas rutin trihydrate by Roth, Germany. ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tris(2-pyridyl)-s-triazine), Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), caffeine were purchased from Sigma-Aldrich, USA. All the chemicals were of analytical grade.
Sodium acetate anhydrous
Manufactured by Chempur
Sourced in Poland, Germany, Austria
Sodium acetate anhydrous is a chemical compound with the formula CH3COONa. It is a white, crystalline solid that is soluble in water and alcohol. The primary function of sodium acetate anhydrous is as a buffer and pH adjuster in various applications, such as in analytical chemistry, biotechnology, and food processing.
Automatically generated - may contain errors
Lab products found in correlation
Potassium persulfate, by Chempur (6 mentions)
Sodium carbonate anhydrous, by Chempur (5 mentions)
Folin ciocalteu reagent, by Merck Group (5 mentions)
Gallic acid, by Merck Group (5 mentions)
Hydrochloric acid (hcl), by Chempur (5 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (4 mentions)
Trolox, by Merck Group (4 mentions)
Iron 2 sulfate heptahydrate, by Merck Group (4 mentions)
Acetic acid, by Chempur (4 mentions)
Methanol, by Chempur (4 mentions)
Copper 2 chloride dihydrate, by Chempur (4 mentions)
Iron 3 chloride hexahydrate, by Chempur (4 mentions)
Neocuproine, by J&K Scientific (4 mentions)
Sodium hydroxide (naoh), by Chempur (3 mentions)
Sodium nitrite, by Chempur (3 mentions)
Aluminum chloride hexahydrate, by Chempur (3 mentions)
Rutin trihydrate, by Carl Roth (3 mentions)
Folin ciocalteu phenol reagent, by Merck Group (2 mentions)
Potassium acetate, by Chempur (2 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (2 mentions)
10 protocols using sodium acetate anhydrous
1
Spectrophotometric Analysis of Antioxidant Compounds
2
Antioxidant Potential of Medicinal Plants
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The following amino acids were used for the synthesis: l -threonine (Thr, >99 wt%, pure), l -methionine (Met, >99 wt%, pure), l -arginine (Arg, >99 wt%, pure), l -isoleucine (Ile, >99 wt%, pure), purchased from Roth, and triethanolamine (TEA) (>99 wt%, pure) from Acros Organics. DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tris(2-pyridyl)-s-triazine) and Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid) were purchased from Sigma-Aldrich (USA). Folin–Ciocalteu phenol reagent, iron(ii ) sulfate heptahydrate and gallic acid were obtained from Merck (Germany), whereas ethanol, 99.5% acetic acid, sodium acetate anhydrous, 36% hydrochloric acid, iron(iii ) chloride hexahydrate, copper(ii ) chloride dihydrate, and sodium carbonate anhydrous were obtained from Chempur (Poland). Neocuproine was delivered by J&K Scientific (Germany).
The research plant material consisted of dry and cut L. clavatum herbs (Dary Natury, Poland), C. islandica (Kawon, Poland) and D. fullonum leaves (NatVita, Poland).
The research plant material consisted of dry and cut L. clavatum herbs (Dary Natury, Poland), C. islandica (Kawon, Poland) and D. fullonum leaves (NatVita, Poland).
3
Antioxidant Capacity Assays: Reagents and Protocols
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2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ), 3,4-dihydroxybenzoic acid, chlorogenic acid, and caffeic acid were purchased from Sigma Aldrich (Poznań, Poland); Folin–Ciocalteu reagent, gallic acid, 4 hydroxybenzoic acid, disodium phosphate, and potassium dihydrogen phosphate from Merck, Darmstadt (Germany); sodium acetate anhydrous, potassium persulfate, potassium acetate, 99.5% acetic acid, 36% hydrochloric acid, sodium chloride, potassium chloride, ethanol, and methanol were from Chempur (Piekary Śląskie, Poland), whereas acetonitrile for HPLC was from J.T. Baker (the Netherlands). All reagents were of analytical grade.
4
Antioxidant Capacity Assays Protocol
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DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6--tris(2-pyridyl)-s-triazine), ABTS (2,2′-azino-bis(3--ethylbenzothiazoline-6-sulfonic acid) and Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxy-lic acid) were purchased from Sigma-Aldrich, USA; gallic acid, Folin-Ciocalteu reagent and iron(II) sulfate heptahydrate from Merck, Germany; rutin trihydrate from Roth, Germany; ferrozine from Serva Electrophoresis, Germany; neocuproine from J&K Scientific, Germany. Methanol, potassium persulfate, 36% hydrochloric acid, iron(III) chloride hexahydrate, 99.5% acetic acid, sodium acetate anhydrous, sodium carbonate anhydrous, copper(II) chloride dihydrate, iron(II) chloride tetrahydrate, potassium chloride, sodium nitrite, aluminum chloride hexahydrate and sodium hydroxide were obtained from Chempur, Poland; ethanol from Linegal Chemicals, Poland. All reagents were of analytical grade.
5
Antioxidant Capacity Assays Protocol
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DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6--tris(2-pyridyl)-s-triazine), ABTS (2,2′-azino-bis(3--ethylbenzothiazoline-6-sulfonic acid) and Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxy-lic acid) were purchased from Sigma-Aldrich, USA; gallic acid, Folin-Ciocalteu reagent and iron(II) sulfate heptahydrate from Merck, Germany; rutin trihydrate from Roth, Germany; ferrozine from Serva Electrophoresis, Germany; neocuproine from J&K Scientific, Germany. Methanol, potassium persulfate, 36% hydrochloric acid, iron(III) chloride hexahydrate, 99.5% acetic acid, sodium acetate anhydrous, sodium carbonate anhydrous, copper(II) chloride dihydrate, iron(II) chloride tetrahydrate, potassium chloride, sodium nitrite, aluminum chloride hexahydrate and sodium hydroxide were obtained from Chempur, Poland; ethanol from Linegal Chemicals, Poland. All reagents were of analytical grade.
6
Comprehensive Antioxidant Analysis Protocol
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2,4,6-tripyridyl-s-triazine (TPTZ), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzo--thiazoline-6-sulphonic acid) (ABTS) were purchased from Sigma-Aldrich, USA; iron(III) chloride hexahydrate, Folin-Ciocalteu phenol reagent -from Merck, Darmstadt, Germany; 99.5% acetic acid, sodium acetate anhydrous, potassium persulfate, 36% hydrochloric acid, sodium carbonate anhydrous, ascorbic acid and applied solvents, i.e. ethanol, methanol, acetone and petroleum ether, were from Chempur, Piekary Śląskie, Poland. All reagents were of analytical grade.
7
Antioxidant Capacity Determination Methods
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The chemicals 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ) were purchased from Sigma Aldrich (Poland). Folin–Ciocalteu reagent was supplied by Merck (Darmstadt, Germany). Anhydrous sodium acetate, potassium persulfate, potassium acetate, ferric chloride, 36% hydrochloric acid, and ethyl alcohol were obtained from Chempur (Piekary Śląskie, Poland). All reagents were of analytical grade.
8
Analytical Procedure for Asparagine and Carbohydrates
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Asparagine (99%) was purchased from Sigma-Aldrich, carbohydrates (>98%) (glucose, fructose, sucrose) from Fluka, acrylamide (>99%) from Merck (Darmstadt, Germany), 2,3,3-d3-acrylamide (>98%) from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA), from Sigma- Aldrich (St. Louis, MO, USA), ethyl acetate and methanol (both of HPLC-grade) from J. T. Baker (Deventer, The Netherlands), and HPLC-grade acetonitrile from Scharlab (Barcelona, Spain). Hexane (99%), Br (99.5%), anhydrous sodium acetate (>99%), bromine water, formic acid, Carrez I, and Carrez II were from Chempur (Piekary Śląskie, Poland). Ultra-purified water (resistivity 18.2 MΩ cm−1) was obtained using Milli-Q Plus Technology, Millipore (Bedford, MA, USA). C 18-E (55μm, 70Å, 6 mL, 500 mg) SPE cartridges were purchased from Shim-Pol (Izabelin, Poland).
9
Synthesis of Alkyl Halide Derivatives
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Naringenin, iodomethane, iodoethane, 1-iodopentane and 1-iodododecane were purchased from Sigma-Aldrich Chemical Co. (Steinheim, Germany), hydroxylamine hydrochloride from LOBA Feinchemie GmbH (Fischamed, Austria), anhydrous sodium acetate and potassium carbonate from Chempur (Piekary Śląskie, Poland). Anhydrous solvents were prepared according to standard procedures. All organic solvents were of analytical grade.
10
Synthesis of Naringenin Derivatives
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Naringenin and 1-iodohexane were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA); hydroxylamine hydrochloride was purchased from LOBA Feinchemie GmbH (Fischamed, Austria); anhydrous sodium acetate and potassium carbonate were purchased from Chempur (Piekary Śląskie, Poland). Anhydrous solvents for reactions (acetone and ethanol) were prepared according to the standard procedures. All organic solvents were of analytical grade.
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