Human svcam 1 cd106 quantikine elisa kit

As markers of hemodynamic status and tissue oxygenation, blood pressure, heart and respiratory rates, Glasgow Coma Scale score (GCS), body temperature, and urine output were monitored before (T0) and after 15 and then 30 minutes, every hour until the hour 6, and then 9, 12, 18, and 24 hours after fluid administration.
Fluid infusion and output were measured and documented. Laboratory blood/plasma parameters including hemoglobin, hematocrit, thrombocyte and leukocyte counts, electrolytes (sodium, potassium, chloride), plasma osmolality, lactate, glucose, albumin, total protein concentrations, SGOT, SGPT, creatinine, venous gas analysis, prothrombin (PT), activated partial thromboplastin time (aPTT), fibrinogen levels, and D-dimer were determined after 0, 6, 12, and 24 hours. sVCAM-1 values were measured after 0, 24, and 48 hours with the ELISA method (R&D Systems, Human sVCAM-1/CD106 Quantikine ELISA Kit, catalog number DVC00). Any adverse events or serious adverse events that occurred during the study period were recorded and reported for all included patients. All concomitant medications and their complement, including dosages, were also documented.

Pharmacodynamic assays were performed to assess the activity of ASP5094 on the known downstream effectors of α9 in RA of TNF-α, MMP-3, and IL-6, which were measured at baseline and each scheduled visit. These serum concentrations were measured by commercially available kits as follows: TNF-α; ELISA, Quantikine HS ELISA (R&D Systems), MMP-3; LTIA, Panaclear MMP-3: Latex (Sekisui Medical), IL-6; CLEIA, IL-6 LPG Immunoreaction Cartridges (Fujirebio). Levels of putative α9 ligands tenascin (TNC)-C (large variant of tenascin-C, termed FNIII-B variant), vascular cell adhesion molecule (VCAM)-1, and osteopontin (OPN; full-length and thrombin-cleaved) were determined at baseline and week 12/discontinuation and week 16/discontinuation, then 4 weeks later for exploratory evaluation of ASP5094 on potential biomarkers. Plasma concentrations of TNC-C, VCAM-1, and OPN were measured by a commercially available ELISA kit as follows: TNC-C; Tn-C large [FNIII-B] assay kit (Immuno-Biological Laboratories), VCAM-1; Human sVCAM1/CD106 Quantikine ELISA kit; (R&D Systems), OPN full-length; Human osteopontin Quantikine ELISA kit (R&D Systems), cleaved-OPN; Human osteopontin N-half assay kit (Immuno-Biological Laboratories).