Standard and samples were separated by HPAEC and detected by pulsed amperometric detection (Dionex) using a CarboPack PA10 (2 × 250 mm) column (LCPackings). Acidic GlcA and GalA were eluted by 10 mM NaOH and 10 mM CH3COONH4 with 1 ml/min flow rate at a retention time of 25 min.
Pulsed amperometric detection
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Pulsed amperometric detection is a technique used in analytical chemistry to quantify the presence and concentration of electroactive analytes in a sample. It involves the application of a series of electrical pulses to an electrode, which results in the oxidation or reduction of the analyte, generating a current that is proportional to the analyte's concentration. This technique is commonly used in high-performance liquid chromatography (HPLC) and ion chromatography for the detection and quantification of various compounds, such as carbohydrates, amino acids, and electroactive ions.
Automatically generated - may contain errors
3 protocols using pulsed amperometric detection
1
Carbohydrate Analysis by HPAEC-PAD
2
Wheat Straw Compositional Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total acid hydrolysis of the as-received wheat straw for sugar analysis was carried out by immersion in 12 M sulphuric acid for 2 hours at 35°C, then 1 M sulphuric acid for 2 hours at 98°C [9 (link)]. Analysis of soluble sugar monomers was by high-performance anion exchange chromatography with pulsed amperometric detection (Dionex, Camberley, UK), using a CarboPac PA20 column under isocratic conditions, with 10 mM NaOH as the mobile phase at a working flow rate of 0.5 ml/min. Glucose, xylose, arabinose and galactose were used as standards with mannitol as internal standard. Analysis of lignin in the as-received straw was carried out by extraction using acetyl bromide in water/dioxane solvent, followed by measurement of absorbance at 280 nm [9 (link)]. Lignin quantification was performed by calibration using a low sulphate lignin reference material. The amount of organic extractable material in as-received straw was determined by Soxhlet extraction for 18 hours in pure ethanol. The extracted mass was determined by weight after rotary evaporation of the liquor followed by vacuum drying [35 ]. All compositional values were calculated on a dry weight basis, summarised in Table 1 .
3
Quantification of Carbohydrates via Ion Chromatography
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An ICS-3000 ion chromatography
HPLC system equipped
with a CarboPac PA-1 column (2 × 250 mm) in combination with
a CarboPac PA-guard column (2 × 25 mm) at 22 °C and a pulsed
electrochemical detector (PAD) in pulsed amperometric detection mode
(Dionex) at 30 °C was used. A flow rate of 0.25 mL min–1 was used, and the column was equilibrated with water. The following
gradient was used: 0–25 min H2O, 25–65 min
at 0–0.045 M NaOH, 65–70 min at 0.045 M NaOH-1 M sodium
acetate in 0.1 M NaOH, 70–75 min at 1 M sodium acetate in 0.1
M NaOH, 75–75.1 min 1 M sodium acetate in 0.1 M NaOH-0.1 M
NaOH, 75.1–80 min 0.1 M NaOH, and 80–95 min H2O. Post column addition was used for increasing the PAD signal by
0.5 M NaOH at a flow rate of 0.15 mL min–1.
HPLC system equipped
with a CarboPac PA-1 column (2 × 250 mm) in combination with
a CarboPac PA-guard column (2 × 25 mm) at 22 °C and a pulsed
electrochemical detector (PAD) in pulsed amperometric detection mode
(Dionex) at 30 °C was used. A flow rate of 0.25 mL min–1 was used, and the column was equilibrated with water. The following
gradient was used: 0–25 min H2O, 25–65 min
at 0–0.045 M NaOH, 65–70 min at 0.045 M NaOH-1 M sodium
acetate in 0.1 M NaOH, 70–75 min at 1 M sodium acetate in 0.1
M NaOH, 75–75.1 min 1 M sodium acetate in 0.1 M NaOH-0.1 M
NaOH, 75.1–80 min 0.1 M NaOH, and 80–95 min H2O. Post column addition was used for increasing the PAD signal by
0.5 M NaOH at a flow rate of 0.15 mL min–1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!