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2 protocols using thccooh gluc

1

Analytical Method for Cannabis Compounds

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THC, 11-OH-THC, THCCOOH, THCCOOH-gluc, CBD, CBN, d3-THC, d3-11-OH-THC, d9-THCCOOH, d3-CBD, d3-CBN and d3-THCCOOH-gluc (d3-THCCOOH-gluc) were purchased from Cerilliant (Round Rock, TX, USA). CBG was obtained from Restek (Bellefonte, PA, USA), THCAA was from Lipomed (Arlesheim, Switzerland), THCVCOOH and THC-gluc from ElSohly Laboratories (Oxford, MS, USA) and THCV from RTI International (Research Triangle Park, NC, USA). Acetonitrile, ammonium acetate and formic acid (LC-MS grade) were acquired from Sigma Aldrich (St. Louise, MO, USA) and methanol (LC-MS grade) from Fischer Scientific (Fair Lawn, NJ, USA). Water was purified in-house by an ELGA Purelab Ultra Analytic purifier (Siemens Water Technologies, Lowell, MA, USA). WAX-S tips (DPX Labs, Columbia, SC, USA) were used to extract samples, and a Kinetex C18 2.1 × 50 mm, 2.6 μm column combined with a 2.1 × 2 mm guard column of identical phase (Phenomenex Inc., Torrance, CA, USA) accomplished analyte separation.
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2

Comprehensive Cannabinoid Standard Preparation

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Stocks of 1 mg/mL of CBD, THC, CBN, 11-OH-THC, CBG, THCV, THCA-A, and THCCOOH-D 3 as well as 100 μg/mL solutions of THCCOOH, THCCOOH-gluc, CBD-D 3 , THC-D 3 , CBN-D 3 , and 11-OH-THC-D 3 were purchased from Cerilliant (Round Rock, TX, USA). A 10-μg/mL THCgluc stock was obtained from ElSohly (Oxford, MS, USA).
Optima LC/MS grade acetonitrile, methanol, 2-propanol, formic acid (FA), and ammonium acetate were obtained from Fisher Scientific (Hampton, NH, USA). Oasis PRiME HLB 96-well solid-phase extraction (SPE) plates with 30 mg sorbent/well, 96-well collection plates, quick-load glass inserts, silicone/PTFE-treated 96-well square plug cap mats, and amber max recovery autosampler vials were acquired from Waters (Milford, MA, USA). Borosilicate glass tubes were from Fisher Scientific and 18.2 MΩ-cm water was dispensed from a Siemens PureLab Ultra purification system.
Blank WB was created by mixing acid citrate dextrose (ACD)packed red blood cells with ABO-compatible plasma and confirming the absence of detectable cannabinoids. Breath matrix consisted of an unused SensAbues ® electret filter material ("pad") cut into 28 cm 2 squares to match the intake surface area of the SensAbues ® collection device.
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