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Anti rps6s235 6

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Anti-RpS6S235/6 is a laboratory reagent used to detect the phosphorylation of ribosomal protein S6 at serine 235 and serine 236. It is a primary antibody that can be used in various cell-based assays and imaging techniques to analyze the activation status of the mTOR signaling pathway.

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Lab products found in correlation

Anti akts473, by Cell Signaling Technology (2 mentions) Haematoxylin eosin, by Cell Signaling Technology (2 mentions) Dako envision flex high ph, by Agilent Technologies (2 mentions)

2 protocols using anti rps6s235 6

1

Immunohistochemical Quantification of Protein Signaling

Cited 1 time
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Histochemical analysis by Haematoxylin-Eosin, anti-RpS6S235/6, anti-AktS473 (Cell Signalling Technologies) and Pten (51-2400) immunostaining was performed as previously described40 (link),41 (link). Immunohistochemical analysis of AMD1 (Proteintech, dilution 1/100) was performed using DAKO envision Flex High pH (DAKO). The scoring system was based on the quantification of the % of cells negative, low (1+), medium (2+) or high (3+) immunoreactivity. Subsequently, H-score was calculated as follows: H = [percentage of cells 1+] + [2 x (percentage of cells 2+)] + [3 x (percentage of cells 3+)]. Differential H score was calculated as follows: ΔH = H[on treatment] – H[pre-treatment].
Zabala-Letona A., Arruabarrena-Aristorena A., Martín-Martín N., Fernandez-Ruiz S., Sutherland J.D., Clasquin M., Tomas-Cortazar J., Jimenez J., Torres I., Quang P., Ximenez-Embun P., Bago R., Ugalde-Olano A., Loizaga-Iriarte A., Lacasa-Viscasillas I., Unda M., Torrano V., Cabrera D., van Liempd S.M., Cendon Y., Castro E., Murray S., Revandkar A., Alimonti A., Zhang Y., Barnett A., Lein G., Pirman D., Cortazar A.R., Arreal L., Prudkin L., Astobiza I., Valcarcel-Jimenez L., Zuñiga-García P., Fernandez-Dominguez I., Piva M., Caro-Maldonado A., Sánchez-Mosquera P., Castillo-Martín M., Serra V., Beraza N., Gentilella A., Thomas G., Azkargorta M., Elortza F., Farràs R., Olmos D., Efeyan A., Anguita J., Muñoz J., Falcón-Pérez J.M., Barrio R., Macarulla T., Mato J.M., Martinez-Chantar M.L., Cordon-Cardo C., Aransay A.M., Marks K., Baselga J., Tabernero J., Nuciforo P., Manning B.D., Marjon K, & Carracedo A. (2017). mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer. Nature, 547(7661), 109-113.
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2

Immunohistochemical Quantification of Protein Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Histochemical analysis by Haematoxylin-Eosin, anti-RpS6S235/6, anti-AktS473 (Cell Signalling Technologies) and Pten (51-2400) immunostaining was performed as previously described40 (link),41 (link). Immunohistochemical analysis of AMD1 (Proteintech, dilution 1/100) was performed using DAKO envision Flex High pH (DAKO). The scoring system was based on the quantification of the % of cells negative, low (1+), medium (2+) or high (3+) immunoreactivity. Subsequently, H-score was calculated as follows: H = [percentage of cells 1+] + [2 x (percentage of cells 2+)] + [3 x (percentage of cells 3+)]. Differential H score was calculated as follows: ΔH = H[on treatment] – H[pre-treatment].
Zabala-Letona A., Arruabarrena-Aristorena A., Martín-Martín N., Fernandez-Ruiz S., Sutherland J.D., Clasquin M., Tomas-Cortazar J., Jimenez J., Torres I., Quang P., Ximenez-Embun P., Bago R., Ugalde-Olano A., Loizaga-Iriarte A., Lacasa-Viscasillas I., Unda M., Torrano V., Cabrera D., van Liempd S.M., Cendon Y., Castro E., Murray S., Revandkar A., Alimonti A., Zhang Y., Barnett A., Lein G., Pirman D., Cortazar A.R., Arreal L., Prudkin L., Astobiza I., Valcarcel-Jimenez L., Zuñiga-García P., Fernandez-Dominguez I., Piva M., Caro-Maldonado A., Sánchez-Mosquera P., Castillo-Martín M., Serra V., Beraza N., Gentilella A., Thomas G., Azkargorta M., Elortza F., Farràs R., Olmos D., Efeyan A., Anguita J., Muñoz J., Falcón-Pérez J.M., Barrio R., Macarulla T., Mato J.M., Martinez-Chantar M.L., Cordon-Cardo C., Aransay A.M., Marks K., Baselga J., Tabernero J., Nuciforo P., Manning B.D., Marjon K, & Carracedo A. (2017). mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer. Nature, 547(7661), 109-113.
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