Goat α rabbit igg hrp

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Goat α-rabbit IgG-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and quantify the presence of rabbit immunoglobulin G (IgG) in various immunoassay applications.

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Lab products found in correlation

Goat α mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) Anti β tubulin, by Merck Group (1 mentions) α bcl xl, by Cell Signaling Technology (1 mentions) Mouse monoclonal β actin, by Merck Group (1 mentions)

Close spelling variants of this product

Goat α-rabbit-HRP IgG Rabbit Rabbit IgGs

2 protocols using goat α rabbit igg hrp

Western Blot Protein Detection

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Extracted protein was probed with anti-ALG-1 (1:5000, Pierce, PA1-031) and anti-β-tubulin (1:5000, Sigma-Aldrich, T4026) primary antibodies followed by goat α-rabbit IgG-HRP (1:5000, Santa Cruz Biotechnology, sc-2004) and goat α-mouse IgG-HRP (1:5000, Santa Cruz Biotechnology, sc-2031) secondary antibodies.

Inukai S., Pincus Z., de Lencastre A, & Slack F.J. (2018). A microRNA feedback loop regulates global microRNA abundance during aging. RNA, 24(2), 159-172.

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Protein Expression Quantification by SDS-PAGE and Western Blotting

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SDS-PAGE and western blotting were performed as previously described [73 (link)]. Primary antibodies used included: rabbit polyclonal α-Bim (Millipore), rabbit polyclonal α-Mcl-1 (Enzo, Farmingdale, NY, USA), hamster monoclonal α-Bcl-2 (BD Biosciences, San Jose, CA, USA), rabbit polyclonal α-Bcl-x_L (Cell Signaling, Danvers, MA, USA), rabbit polyclonal α-Lasu1 (Bethyl Laboratories, Montgomery, TX, USA), and mouse monoclonal β-actin (Sigma). The following secondary antibodies were used: goat α-rabbit IgG-HRP (Santa Cruz Biotechnology, Dallas, TX, USA), mouse α-Armenian and Syrian hamster IgG-HRP (BD Biosciences), and sheep α-mouse IgG-HRP (GE Healthcare, Little Chalfont, Buckinghamshire, UK).

Conage-Pough J.E, & Boise L.H. (2018). Phosphorylation alters Bim-mediated Mcl-1 stabilization and priming. The FEBS journal, 285(14), 2626-2640.

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