Anti-LC3A/B (Cat. 1274), anti-Beclin 1 (Cat. 3495), and ATG7 (Cat. 8558) were purchased from Cell Signaling Technology (Danvers, MA). Both anti-β-actin (Cat. SAB2100037), anti-mTOR (Cat. PLA0114), and anti-Flag (F3165) antibodies were purchased from Sigma (St. Louis, MO). The rabbit polyclonal Polγ (Cat. PA5–75881) was purchased from Thermo Scientific (Waltham, MA). Anti-Raptor (Cat. 05–1470) and anti-Rictor (Cat. 05–1471) antibodies were purchased from Millipore (Temecula, CA). Polγ siRNA (Cat. Sc-155884) and the lentiviral Polγ shRNA plasmid (Sc-155884-SH) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). PCR ampli-fied full-length Polγ cDNA was sucloned into pEGFP-N1-Flag expression vector (Addgene, Cat. 60360), and the pEGFP-LC3 plasmid was a kind gift of Dr. Xianglin Shi, University of Kentucky (Lexington, KY). MnTnBuOE-2-PyP5+ (MnP) was the kind gift of Dr. Ines Batinic-Haberle, Duke University School of Medicine (Durham, NC). 3-Methyle adenine (3-MA, Cat. tlrl-3ma) and Bafilomycin A1 (Cat. tlrl-baf1) were purchased from InvivoGen (San Diego, CA). 10-nonylacridine orange bromide (NAO) was purchased from Thermo Fisher (Cat. A1372) All other chemicals were purchased from Sigma unless otherwise specified.
Bafilomycin a1 cat tlrl baf1
Manufactured by InvivoGen
Bafilomycin A1 (Cat. tlrl-baf1) is a macrolide antibiotic that functions as a selective and potent inhibitor of vacuolar-type H+-ATPase (V-ATPase). V-ATPase is a proton pump that plays a crucial role in the acidification of intracellular organelles. Bafilomycin A1 inhibits this process, leading to the disruption of pH homeostasis within the cell.
Automatically generated - may contain errors
Lab products found in correlation
Anti beclin 1, by Cell Signaling Technology (2 mentions)
Anti lc3a b, by Cell Signaling Technology (2 mentions)
Anti rictor, by Merck Group (2 mentions)
Polγ sirna, by Santa Cruz Biotechnology (2 mentions)
Polγ shrna plasmid sc 155884 sh, by Santa Cruz Biotechnology (2 mentions)
Pegfp n1 flag expression vector, by Addgene (2 mentions)
10 nonylacridine orange bromide nao, by Thermo Fisher Scientific (2 mentions)
Anti raptor, by Merck Group (2 mentions)
Anti flag f3165, by Merck Group (2 mentions)
2 protocols using bafilomycin a1 cat tlrl baf1
1
Autophagy Regulation in Mitochondrial Dynamics
2
Autophagy Pathway Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-LC3A/B (Cat. 1274), anti-Beclin 1 (Cat. 3495), and ATG7 (Cat. 8558) were purchased from Cell Signaling Technology (Danvers, MA). Both anti-β-actin (Cat. SAB2100037), anti-mTOR (Cat. PLA0114), and anti-Flag (F3165) antibodies were purchased from Sigma (St. Louis, MO). The rabbit polyclonal Polγ (Cat. PA5-75881) was purchased from Thermo Scientific (Waltham, MA). Anti-Raptor (Cat. 05–1470) and anti-Rictor (Cat. 05–1471) antibodies were purchased from Millipore (Temecula, CA). Polγ siRNA (Cat. Sc-155884) and the lentiviral Polγ shRNA plasmid (Sc-155884-SH) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). PCR amplified full-length Polγ cDNA was sucloned into pEGFP-N1-Flag expression vector (Addgene, Cat. 60360), and the pEGFP-LC3 plasmid was a kind gift of Dr. Xianglin Shi, University of Kentucky (Lexington, KY). MnTnBuOE-2-PyP5+ (MnP) was the kind gift of Dr. Ines Batinic-Haberle, Duke University School of Medicine (Durham, NC). 3-Methyle adenine (3-MA, Cat. tlrl-3ma) and Bafilomycin A1 (Cat. tlrl-baf1) were purchased from InvivoGen (San Diego, CA). 10-nonylacridine orange bromide (NAO) was purchased from Thermo Fisher (Cat. A1372) All other chemicals were purchased from Sigma unless otherwise specified.
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