Anlotinib hydrochloride capsules (12 mg/capsule) were obtained from Zhengda Tianqing Company (batch number: H20180004). HPLC-grade methanol, ammonium hydroxide, ammonium acetate and acetonitrile were purchased from CNW Technologies. The internal standard, 2-chloro-L-phenylalanine, was obtained from Shanghai Hengbai Biotech Co., Ltd.
Ammonium hydroxide
Manufactured by CNW Technologies
Sourced in Germany, China
Ammonium hydroxide is a chemical compound with the formula NH4OH. It is a colorless, aqueous solution that is commonly used as a laboratory reagent. Ammonium hydroxide has a strong, pungent odor and is a weak base.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium acetate, by CNW Technologies (6 mentions)
Acetonitrile, by CNW Technologies (5 mentions)
Methanol, by CNW Technologies (4 mentions)
Hplc grade methanol, by CNW Technologies (2 mentions)
Formic acid, by CNW Technologies (2 mentions)
Malondialdehyde, by Nanjing Jiancheng (1 mentions)
Hplc grade acetonitrile, by CNW Technologies (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
8 protocols using ammonium hydroxide
1
Anlotinib Hydrochloride Capsules Analysis
2
Quantitative Analysis of Indigo Compounds
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Chemicals: indigo, indirubin and (R,S)- epigoitrin (National Institute for Food and Drug Control, China).
Reagents: Methanol, acetonitrile, ammonium acetate, ammonium hydroxide (CNW Technologies, LC-MS grade), 2-Chloro-L-phenylalanine (Shanghai Hengbai Biotech Co., Ltd).
Reagents: Methanol, acetonitrile, ammonium acetate, ammonium hydroxide (CNW Technologies, LC-MS grade), 2-Chloro-L-phenylalanine (Shanghai Hengbai Biotech Co., Ltd).
3
Screening Serum Biomarkers of Chronic Renal Failure
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27 serum samples were collected from patients with RF (RF), and corresponding healthy control serum were obtained from 27 healthy people (HC). All of the serum samples were obtained from Yinchuan Weike Renal Specialty Hospital, from the year 2018 to 2019. Detailed information of the serum samples are listed in Table 1 and Table 2 , and the clinical characteristics of patients are presented in Supplementary Table S1 . In this study, patients at CKD stage 5 with an estimated glomerular filtration rate (eGFR) < 15 mL/min/1.73 m2 (chronic kidney disease, CKD 5, n = 27) and HC participants without acute inflammatory disease were selected for screening serum biomarkers of chronic renal failure. The serum samples were packaged and stored at −80°C for later analysis. Methanol, acetonitrile, ammonium acetate, and ammonium hydroxide were all chromatography grade (CNW Technologies, Germany).
4
Quantification of Oxidative Stress Markers
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Chemical carbon tetrachloride (CCl4) was purchased from Tianjin Tianli Chemical Reagent Co., Ltd. (Tianjin, China). Silymarin was obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). The diagnostic kits specific for necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) were obtained from the Jiancheng Institute of Biotechnology (Nanjing, China). Chromatography-grade methanol, acetonitrile, ammonium hydroxide, and ammonium acetate were provided by CNW Technologies (Shanghai, China). All of the other chemicals and reagents used in the experiments were of analytical grade.
5
Metabolomic Analysis of Botanical Samples
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Reagents including methanol, acetonitrile, ammonium acetate, ammonium hydroxide, and formic acid were purchased from CNW Technologies (ANPEL Laboratory Technologies, Inc. Shanghai, China). The internal standards of 2-chloro-L-phenylalanine was purchased from Shanghai Hengbai Biotech Co., Ltd. (Shanghai, China), and azadirachtin (>90%) were provided by Associate Professor Yongqing Tian at the South China Agricultural University.
Major instruments used in this study included ultra-high performance liquid chromatography (UHPLC) (Agilent Technologies, Santa Clara, CA, USA) coupled with Q Exactive Focus mass spectrometer (MS) (Thermo Fisher Scientific, Waltham, MA, USA), centrifuge, scales, grinding mill, water purification, ultrasound instrument, and column (ACQUITY UPLC HSS T3 1.7 μm, 2.1 × 100 mm).
Major instruments used in this study included ultra-high performance liquid chromatography (UHPLC) (Agilent Technologies, Santa Clara, CA, USA) coupled with Q Exactive Focus mass spectrometer (MS) (Thermo Fisher Scientific, Waltham, MA, USA), centrifuge, scales, grinding mill, water purification, ultrasound instrument, and column (ACQUITY UPLC HSS T3 1.7 μm, 2.1 × 100 mm).
6
Bovine lactoferrin purification and characterization
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Bovine lactoferrin (bLF) (purity ≥ 96.3%, iron saturation 15%) was obtained from Westland Cooperative Dairy Co. Ltd. (Hokitika, New Zealand). High-performance liquid chromatography (HPLC)-grade acetonitrile, methanol, ammonium acetate, ammonium hydroxide, and formic acid were purchased from CNW Technologies GmbH (Düsseldorf, Germany). 2-chloro-L-phenylalanine (purity ≥ 98%) was purchased from Shanghai Hengbai Biotechnology Co. Ltd. (Shanghai, China). The other chemicals used in this study were of analytical grade.
7
HPLC Analysis of L-2-Chlorophenylalanine
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All chemicals and solvents were of analytical or HPLC grade. Acetonitrile, methanol, ammonium hydroxide, and ammonium acetate were purchased from CNW Technologies GmbH (Düsseldorf, Germany). L-2-chlorophenylalanine was obtained from Shanghai Hengchuang Biotechnology Co., Ltd. (Shanghai, China).
8
Dexamethasone Injection Quantification
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HPLC grade methanol, acetonitrile, ammonium acetate, and ammonium hydroxide were purchased from CNW Technologies (CNW, Germany). All aqueous solutions were prepared with ultrapure water produced by a Milli-Q water purification system (Millipore Corp., Billerica, MA, USA). Dexamethasone solution for injection was purchased from Shanghai Xinyi Pharmaceutical Co (Shanghai, China).
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