Rats were sacrificed using an overdose (100 mg/kg) of pentobarbital sodium salt (Merck KGaA, Darmstadt, Germany) and claws were fixed in 4.0% formaldehyde for 12 h at room temperature prior to decalcification in 10% EDTA solution at room temperature for ~30 days. Paraffin sections of the claws (7 µm) were stained using a hematoxylin and eosin (H&E) kit (Nanjing Jiancheng Technology Co., Ltd., Nanjing, China) to identify synovial inflammation and bone destruction. A leukocyte acid phosphatase staining (TRAP) kit (Sigma-Aldrich, Inc., Merck KGaA) was used to detect osteoclasts, according to the manufacturer's instructions. Sections were observed using an Olympus BX53 microscope (Olympus Corporation, Tokyo, Japan).
H e kit
Manufactured by Nanjing Jiancheng
Sourced in China
The H&E kit is a laboratory tool used for the preparation and staining of tissue samples. It contains reagents and materials necessary for the hematoxylin and eosin (H&E) staining method, which is a widely used histological technique to visualize cellular structures under a microscope.
Automatically generated - may contain errors
4 protocols using h e kit
1
Histological Analysis of Rat Claws
2
Cell Morphology Assessment with HE Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After cultured for 2, 4 or 6 days, cells were washed three times with PBS and fixed in 95% alcohol for 30 minutes. Cells were then washed with PBS again and stained with hematoxylin and eosin (HE) kit (Jiancheng Biotech, China). Finally, cells were observed and photographed utilizing an inverted phase contrast microscope (Zeiss Corporation, Germany).
3
Histological Analysis of Myocardial Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the myocardial tissue was fixed with tissue fixative for 24-72 hours, it was removed from the fixative and washed with running water for 1 hour. According to the method reported in the literature, the heart tissue was sliced, and the H&E kit (Jiancheng, Nanjing, China) was used for staining. After mounting with neutral resin, the image was observed under a microscope (Thermo Fisher Scientific, Waltham, MA, USA).
4
Evaluation of Pancreatitis in Rat Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SD rats (adult male, SPF grade, weight 250 ± 10 g) were obtained from Chengdu Dashuo Laboratory Animal Co., Ltd, and care was provided according to the guidelines of the Institutional of Animal Care and Use Committee. The HE kit, potassium test kit, calcium test kit, amylase test kit and lipase test kit were produced from Nanjing Jiancheng Bioengineering Institute. The TUNEL kit was purchased from Promega Company. The enzyme-linked immunosorbent assay (ELISA) kit for the detection of inflammatory factors was purchased from Boster Biological Technology co., Ltd.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!