The largest database of trusted experimental protocols

Etodolac

Manufactured by Cayman Chemical

Etodolac is a laboratory reagent used for research purposes. It is a nonsteroidal anti-inflammatory drug (NSAID) that functions as a cyclooxygenase (COX) inhibitor. Etodolac is commonly used in studies involving inflammation, pain, or related biological processes.

Automatically generated - may contain errors

2 protocols using etodolac

1

Amnion Biomechanics Under Inflammatory Conditions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Then 3- ×3-cm sections of amnion from CS-term pregnancies were prepared from
the region measuring 5 cm away from the edge of the placental disk and were incubated
in media (no drug control), 10 ng/mL TNF-α (Peprotech), 0.5%
ethacridine (Abcam), 10−5 M Etodolac (Sigma Aldrich, St. Louis,
MO), 10 μM celecoxib (Cayman, Ann Arbor, MI), and 10 ng/mL
TNF-α with 10−5 M Etodolac at
37°C overnight. The treated amnion was placed to cover the end of a
pressurized tube 1 cm in diameter and placed within a DMEM bath. The tube was
connected to a syringe pump, which produced pressurized flow of infused DMEM.
Membrane rupture was signaled by blue dye from the DMEM entering the DMEM bath.
Pressure and time of rupture was recorded. Western blot was performed on all ruptured
membranes.
+ Open protocol
+ Expand
2

Anti-inflammatory Compound Screening in Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
BMDMs were treated with with 0.125 – 0.5 mM indomethacin (Sigma-Aldrich), 0.25 – 1 mM (S)-ibuprofen (Cayman Chemical), 0.125 – 0.5 mM ketoprofen (Sigma-Aldrich), 0.25 – 1 mM Etodolac (Cayman Chemical), or 0.25 – 1 mM naproxen (Cayman Chemical), 0.25–1 mM rofecoxib, 5 mM sulforaphane (Cayman Chemical), 100 μM dimethylfumarate. NSAIDs were diluted in 1% DMSO. Cells were pretreated with NSAID for 1 hour prior to stimulation with 10 ng/mL LPS (L2880, Sigma-Aldrich). LDH assay performed according to manufacturer’s instructions (601170, Cayman Chemical). Reactive oxygen species (ROS) measured using H2DCFDA (D399, ThermoFisher) as described previously (Kavian et al., 2018 (link)). Sodium arsenite (Sigma-Aldrich), naphthalenedione (DMNQ, Cayman Chemical) and phenazine methosulfate (PMS, Cayman Chemical) at the indicated doses were used to induce ROS in BMDM.
MDMs were treated with indicated concentrations of indomethacin (Sigma-Aldrich), ketoprofen (Cayman Chemical), or sulforaphane (Cayman Chemical) for indicated durations.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!