Then 3- ×3-cm sections of amnion from CS-term pregnancies were prepared from
the region measuring 5 cm away from the edge of the placental disk and were incubated
in media (no drug control), 10 ng/mL TNF-α (Peprotech), 0.5%
ethacridine (Abcam), 10−5 M Etodolac (Sigma Aldrich, St. Louis,
MO), 10 μM celecoxib (Cayman, Ann Arbor, MI), and 10 ng/mL
TNF-α with 10−5 M Etodolac at
37°C overnight. The treated amnion was placed to cover the end of a
pressurized tube 1 cm in diameter and placed within a DMEM bath. The tube was
connected to a syringe pump, which produced pressurized flow of infused DMEM.
Membrane rupture was signaled by blue dye from the DMEM entering the DMEM bath.
Pressure and time of rupture was recorded. Western blot was performed on all ruptured
membranes.
the region measuring 5 cm away from the edge of the placental disk and were incubated
in media (no drug control), 10 ng/mL TNF-α (Peprotech), 0.5%
ethacridine (Abcam), 10−5 M Etodolac (Sigma Aldrich, St. Louis,
MO), 10 μM celecoxib (Cayman, Ann Arbor, MI), and 10 ng/mL
TNF-α with 10−5 M Etodolac at
37°C overnight. The treated amnion was placed to cover the end of a
pressurized tube 1 cm in diameter and placed within a DMEM bath. The tube was
connected to a syringe pump, which produced pressurized flow of infused DMEM.
Membrane rupture was signaled by blue dye from the DMEM entering the DMEM bath.
Pressure and time of rupture was recorded. Western blot was performed on all ruptured
membranes.