Aia pack iri

Manufactured by Tosoh

Sourced in Japan

The AIA-PACK IRI is a laboratory equipment product developed by Tosoh. It is designed to perform immunoassay analyses. The core function of the AIA-PACK IRI is to automate the process of immunoassay testing, which involves the detection and measurement of specific analytes in a sample.

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Lab products found in correlation

Cobas 311, by Roche (1 mentions) Aia 900, by Tosoh (1 mentions)

Spelling variants of this product

ST AIA-PACK IRI (3 citations)

6 protocols using aia pack iri

Standardized Glucose Tolerance Test

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In all obese subjects, a standard 2-hour OGTT was performed according to standard procedures, as previously described [10] (link).
HOMA-IR [(Fasting Insulin*Fasting Glucose)/22.5] was calculated as insulin resistance index. Plasma glucose level was determined by using the glucose oxidase method and plasma insulin was measured with two-site immunoenzymometric assay (AIA-PACK IRI; Tosoh, Tokyo, Japan). The limit of detection was 0.5 μU/mL with intra-and interassay coefficients of variation <7% for quality control.

Giannini C. (2020). Asymmetric Dimethylarginine in Obese Youth: Relationship with 24-hour Ambulatory Blood Pressure. Biomedical Journal of Scientific & Technical Research, 31(2).

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Glucose, Insulin, and HbA1c Measurement

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Blood glucose was determined by using the glucose oxidase method, and plasma insulin was measured with twosite immune-enzymometric assay (AIA-PACK IRI; Tosoh, Tokyo, Japan). The lower detection was 0.5 µU/mL with intra-assay and inter-assay CVs of 7% for quality control. HbA1c concentrations were measured using a high-performance liquid chromatography method. The normal range was 4.2-6.0%, with intra-assay CVs of 1.0-1.2% and inter-assay CVs of1.4-1.9% (22) .

Giannini C., Mastromauro C., Chiarelli F, & Mohn A. (2023). Effects of COVID-19 time on the development of pre-impaired glucose tolerance state in children and adolescents with overweight and obesity. International journal of obesity (2005), 47(11).

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Plasma Glucose and Insulin Measurement

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Plasma glucose level was determined by the glucose oxidase method, and plasma insulin was measured with a 2-site immunoenzymometric assay (AIAPACK IRI; Tosoh, Tokyo, Japan). The limit of detection was 0.5 µU/mL with intraassay and interassay coefficients of variation of <7%. HOMA-IR was calculated with the formula: fasting insulin (μU/mL) x fasting glucose (mmol/L)/22.5 [23] .

Chiavaroli V., Marcovecchio M.L., de Giorgis T., Diesse L., Chiarelli F, & Mohn A. (2014). Progression of Cardio-Metabolic Risk Factors in Subjects Born Small and Large for Gestational Age. PLoS ONE, 9(8), e104278.

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Evaluation of Glucose Metabolism

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Serum was analyzed at the University of Missouri Diabetes Diagnostic Laboratory for hemoglobin A1c (HbA1c) (using the Tosoh G8 variant mode ion-exchange HPLC method; Tosoh Biosciences, San Francisco, CA). Insulin and C-peptide were measured using the Tosoh AIA-PACK IRI and ST AIA-PACK C-peptide II reagents on the Tosoh AIA-900 (Tosoh Biosciences, San Francisco, CA). In the OGTT experiment, glucose was measured in fluoride plasma using the Roche GLUC3 hexokinase reagents on a Roche Cobas 311 (Roche Diagnostics, Indianapolis, IN). In the HG clamp experiment, plasma glucose was measured every 5 minutes at the bedside in the CRC using the YSI 2300 Stat Plus (YSI Inc., Yellow Springs, OH).

Stahlhut R.W., Myers J.P., Taylor J.A., Nadal A., Dyer J.A, & vom Saal F.S. (2018). Experimental BPA Exposure and Glucose-Stimulated Insulin Response in Adult Men and Women. Journal of the Endocrine Society, 2(10), 1173-1187.

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Serum Glucose and Insulin Assays

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Serum glucose levels were determined using the glucose oxidase method, and serum insulin levels were measured by the two-site immunoenzymetric assay (AIA-PACK IRI; Tosoh, Tokyo, Japan).

Di Pietro N., Marcovecchio M.L., Di Silvestre S., de Giorgis T., Cordone V.G., Lanuti P., Chiarelli F., Bologna G., Mohn A, & Pandolfi A. (2017). Plasma from pre-pubertal obese children impairs insulin stimulated Nitric Oxide (NO) bioavailability in endothelial cells: Role of ER stress. Molecular and Cellular Endocrinology, 443, 52-62.

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Insulin Resistance Assessment Protocol

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The Homeostasis Model Assessment (HOMA) was used to evaluate insulin resistance using the following formula: fasting serum insulin (uU/mL) × fasting plasma glucose (mmol/L) / 22.5 [16 (link)].
Participants were classified as insulin sensitive if their HOMA score was ≤ 2.6, with a HOMA score > 2.6 used to denote insulin resistance [17 (link)]. The Tosoh AIA-PACK IRI, a two-site immunoenzymometric assay, was used to measure blood insulin levels, with glucose measured spectrophotometrically. Details of the assessment of insulin and glucose have been previously described [18 ].

Loprinzi P.D, & Abbott K. (2014). Physical activity and total serum bilirubin levels among insulin sensitive and insulin resistant U.S. adults. Journal of Diabetes and Metabolic Disorders, 13, 47.

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