Salirasib (5 μM, #SML1166; Sigma), Sorafenib (5 μM, #8705) and U0126 (20 μM, #9903; Cell Signaling Technology), Gefitinib (10 μM, Iressa, #S1025; Selleck-Chem), GI254023X (20 μM, #3995) and PF573228 (10 μM, #3239; Tocris), and GW280264X (20 μM, AOB3632; Aobious) were all used at the indicated concentrations. Monoclonal anti-B5 (VMC-20) was a kind gift of Gary H. Cohen and Roselyn J Eisenberg (University of Pennsylvania)40 (link). Phospho-EGF receptor antibody sampler kit (#9922, containing #4267, 3777, 2237), phospho-Erk1/2 pathway sampler kit (#9911, containing #9427, 9154, 4370, 11989), FAK antibody sampler kit (#9330, containing #3281, 3284, 8556, 13009), alpha-tubulin DM1A antibody (#3873), and anti-rabbit and anti-mouse IgG HRP-linked antibodies (#7074, 7076) were purchased from Cell Signaling Technology and used at 1:1000. Anti-ADAM10 (#ab124695) and anti-ADAM17 (#ab2051) were purchased from Abcam and used at 1:1000. IRDye-coupled secondary antibodies were purchased from Licor and used at 1:10'000. Alexa Fluor-conjugated secondary antibodies and phalloidin were purchased from Invitrogen/Thermo Fischer Scientific and used at 1:1000 or 1:100, respectively.
Phospho egf receptor antibody sampler kit
Manufactured by Cell Signaling Technology
The Phospho-EGF receptor antibody sampler kit is a collection of antibodies that specifically recognize different phosphorylation sites on the epidermal growth factor receptor (EGFR) protein. The kit includes a set of well-characterized antibodies that can be used to study the activation and signaling of EGFR in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Anti rabbit and anti mouse igg hrp linked antibodies, by Cell Signaling Technology (2 mentions)
Anti adam10, by Abcam (2 mentions)
Gefitinib, by Selleck Chemicals (2 mentions)
Sorafenib, by Cell Signaling Technology (2 mentions)
Salirasib, by Merck Group (2 mentions)
Pf573228, by Bio-Techne (2 mentions)
Phospho erk1 2 pathway sampler kit, by Cell Signaling Technology (2 mentions)
Alpha tubulin dm1a antibody, by Cell Signaling Technology (2 mentions)
Fak antibody sampler kit, by Cell Signaling Technology (2 mentions)
Gi254023x, by Bio-Techne (2 mentions)
Anti adam17, by Abcam (2 mentions)
U0126, by Cell Signaling Technology (2 mentions)
Ir dye coupled secondary antibodies, by LI COR (2 mentions)
Gw280264x, by Aobious (2 mentions)
2 protocols using phospho egf receptor antibody sampler kit
1
Characterization of EGFR Signaling Inhibitors
2
Characterization of EGFR Signaling Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Salirasib (5 μM, #SML1166; Sigma), Sorafenib (5 μM, #8705) and U0126 (20 μM, #9903; Cell Signaling Technology), Gefitinib (10 μM, Iressa, #S1025; Selleck-Chem), GI254023X (20 μM, #3995) and PF573228 (10 μM, #3239; Tocris), and GW280264X (20 μM, AOB3632; Aobious) were all used at the indicated concentrations. Monoclonal anti-B5 (VMC-20) was a kind gift of Gary H. Cohen and Roselyn J Eisenberg (University of Pennsylvania)40 (link). Phospho-EGF receptor antibody sampler kit (#9922, containing #4267, 3777, 2237), phospho-Erk1/2 pathway sampler kit (#9911, containing #9427, 9154, 4370, 11989), FAK antibody sampler kit (#9330, containing #3281, 3284, 8556, 13009), alpha-tubulin DM1A antibody (#3873), and anti-rabbit and anti-mouse IgG HRP-linked antibodies (#7074, 7076) were purchased from Cell Signaling Technology and used at 1:1000. Anti-ADAM10 (#ab124695) and anti-ADAM17 (#ab2051) were purchased from Abcam and used at 1:1000. IRDye-coupled secondary antibodies were purchased from Licor and used at 1:10'000. Alexa Fluor-conjugated secondary antibodies and phalloidin were purchased from Invitrogen/Thermo Fischer Scientific and used at 1:1000 or 1:100, respectively.
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