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Ac ietd cho

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Ac-IETD-CHO is a chemical compound that functions as a caspase-8 inhibitor. It is commonly used in research applications to study apoptosis and cell death pathways.

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Lab products found in correlation

Sulprostone, by Cayman Chemical (2 mentions) Forskolin, by Cayman Chemical (2 mentions) 6 bnz camp, by Biolog (2 mentions) Ac yvad cho, by Enzo Life Sciences (2 mentions) Pseudomonas aeruginosa lps, by Merck Group (2 mentions) Naphthol as e phosphate, by Merck Group (2 mentions) Ono ae1 329, by Merck Group (2 mentions) 8 pcpt 2 om camp, by Biolog (2 mentions) Butaprost, by Cayman Chemical (2 mentions) Adenosine triphosphate (atp), by Merck Group (2 mentions) Mitotracker red, by Thermo Fisher Scientific (1 mentions) Z vad fmk, by Promega (1 mentions) Dioc6, by Thermo Fisher Scientific (1 mentions) Z lehd fmk, by BD (1 mentions) Wi 38, by National Centre for Cell Science (1 mentions) Ac devd cho, by Promega (1 mentions) H2dcfda, by Thermo Fisher Scientific (1 mentions) Sybr green master mix, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)

4 protocols using ac ietd cho

1

Inflammatory Signaling Pathway Modulation

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PGE2 (1-1000nM; Cayman Chemicals, Ann Arbor, MI); Forskolin (25μM; Cayman Chemical, Ann Arbor, MI); Pseudomonas aeruginosa LPS (100ng/ml; Sigma-Aldrich, St. Louis, MO); ATP (1μM; Sigma-Aldrich, St. Louis, MO); Caspase-1 inhibitor (10nM Ac-YVAD-CHO; Enzo Life Sciences, Farmingdale, NY); Caspase-8 inhibitor (10nM Ac-IETD-CHO; BD Biosciences, San Jose, CA); CREB inhibitor (100μM Naphthol AS-E phosphate; Sigma-Aldrich, St. Louis, MO); EP2 agonist (1μM Butaprost; Cayman Chemical, Ann Arbor, MI), EP3 agonist (10nM Sulprostone; Cayman Chemical, Ann Arbor, MI) EP4 agonist (500nM ONO-AE1-329; Sigma-Aldrich, St. Louis, MO); Protein Kinase A (PKA) agonist (50μM 6-BNZ-cAMP; Biolog, Hayward, CA); Epac agonist (50μM 8-pcpt-2′-OM-cAMP; Biolog, Hayward, CA).
Martínez-Colón G.J., Taylor Q.M., Wilke C.A., Podsiad A.B, & Moore B.B. (2017). Elevated Prostaglandin E2 Post-Bone Marrow Transplant Mediates Interleukin-1β Related-Lung Injury. Mucosal immunology, 11(2), 319-332.
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2

Inflammatory Signaling Pathway Modulation

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PGE2 (1-1000nM; Cayman Chemicals, Ann Arbor, MI); Forskolin (25μM; Cayman Chemical, Ann Arbor, MI); Pseudomonas aeruginosa LPS (100ng/ml; Sigma-Aldrich, St. Louis, MO); ATP (1μM; Sigma-Aldrich, St. Louis, MO); Caspase-1 inhibitor (10nM Ac-YVAD-CHO; Enzo Life Sciences, Farmingdale, NY); Caspase-8 inhibitor (10nM Ac-IETD-CHO; BD Biosciences, San Jose, CA); CREB inhibitor (100μM Naphthol AS-E phosphate; Sigma-Aldrich, St. Louis, MO); EP2 agonist (1μM Butaprost; Cayman Chemical, Ann Arbor, MI), EP3 agonist (10nM Sulprostone; Cayman Chemical, Ann Arbor, MI) EP4 agonist (500nM ONO-AE1-329; Sigma-Aldrich, St. Louis, MO); Protein Kinase A (PKA) agonist (50μM 6-BNZ-cAMP; Biolog, Hayward, CA); Epac agonist (50μM 8-pcpt-2′-OM-cAMP; Biolog, Hayward, CA).
Martínez-Colón G.J., Taylor Q.M., Wilke C.A., Podsiad A.B, & Moore B.B. (2017). Elevated Prostaglandin E2 Post-Bone Marrow Transplant Mediates Interleukin-1β Related-Lung Injury. Mucosal immunology, 11(2), 319-332.
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3

Fibroblast Cell Line Characterization

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Human embryonic fibroblast lung cell line (WI38) was purchased from the National Centre for Cell Science (Pune, India). Dulbecco's modified Eagle medium (DMEM), fetal bovine serum (FBS) and antibiotic/antimycotic solution and gentamycin were purchased from HyClone, Thermo Fisher Scientific (Waltham, MA). All primary and secondary antibodies were procured from Santa Cruz Biotechnology (Santa Cruz, CA) and Cell Signaling Technology (Beverly, MA). Alexa fluor 660-conjugated anti-goat secondary antibody and FITC-conjugated anti-rabbit, anti-mouse, and anti-goat secondary antibodies were from Invitrogen (Carlsbad, CA) and Millipore (Billerica, MA, USA). The pan caspase inhibitor (Z-VAD-fmk) and the caspase-3/7 inhibitor (Ac-DEVD-CHO) were purchased from Promega (Madison, WI). The caspase-8 inhibitor (Ac-IETD-CHO) and the caspase-9 inhibitor (Z-LEHD-fmk) were purchased from BD Biosciences (San Diego, CA). The JNK inhibitor SP600125 and the p38 inhibitor SB203580 were purchased from Enzo Life Sciences International, Inc. (Plymouth, PA). DiOC6, H2DCF-DA, MitoTracker Red, SYBR Green Master Mix, and TRIzol reagent were procured from Invitrogen, Carlsbad, CA. All other chemicals were from Merck (Mumbai, India and Darmstadt, Germany), Sigma Aldrich (St. Louis, MO), and Himedia (Mumbai, India) unless indicated otherwise.
Roy Chowdhury S., Sengupta S., Biswas S., Sinha T.K., Sen R., Basak R.K., Adhikari B, & Bhattacharyya A. (2014). Bacterial Fucose-Rich Polysaccharide Stabilizes MAPK-Mediated Nrf2/Keap1 Signaling by Directly Scavenging Reactive Oxygen Species during Hydrogen Peroxide-Induced Apoptosis of Human Lung Fibroblast Cells. PLoS ONE, 9(11), e113663.
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4

Cytotoxicity Screening of PCB Congeners and Inhibitors

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Aroclor 1254 and individual congeners (PCB 77, PCB 126, PCB 153, PCB 180) were purchased from VWR International (Radnor, PA, USA). The purity of each PCB congener was higher than 99%, as assayed by gas-chromatography. Thyroid hormone receptor antagonist (1–850), α-Naphthoflavone (α-NF) (AhR inhibitor) and 7-Hydroxyflavone (HF) (CYP1A1 inhibitor) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). cis-diammineplatinum(II)dichloride (Cisplatin), Tumor Necrosis Factor-α (TNF-α), 3,3’,5 triiodothyronine (T3), 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), Cycloheximide (CHX), Colchicine, Hydrogen Peroxide (H2O2) and Ascorbic Acid (vitamin C) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ac-DEVD-CHO (caspase-3 inhibitor) and Ac-IETD-CHO (caspase-8 inhibitor) were purchased from BD Biosciences (San Jose, CA, USA). Ac-LEHD-CHO caspase-9 inhibitor was purchased from Alexis Biochemicals (San Diego, CA, USA). Chemicals were dissolved in dimethyl sulfoxide (DMSO), which was used as a vehicle (0.1% final concentration), in the cellular apoptosis experiments.
Raggi F., Russo D., Urbani C., Sardella C., Manetti L., Cappellani D., Lupi I., Tomisti L., Martino E., Marcocci C, & Bogazzi F. (2016). Divergent Effects of Dioxin- or Non-Dioxin-Like Polychlorinated Biphenyls on the Apoptosis of Primary Cell Culture from the Mouse Pituitary Gland. PLoS ONE, 11(1), e0146729.
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