Total RNA was isolated from HEK293 and HeLa cells via Phenol/Chloroform extraction according to manufacturer’s protocol (Trizol, Thermo Fisher Scientific, MA, US). The BioAnalyzer System was used to measure the quality and quantity of total RNA. (BioAnalyzer RNA Pico Kit, Agilent, CA, USA). 2 μg of total RNA was used for RNA purification with magnetic bead cleanup module (Life Technologies, CA, USA). Purified RNA was ligated with sequencing adapters, reverse transcribed and purified (Ion Total RNA-Seq Kit v2, Life Technologies, CA, USA). Finally, barcodes were added and library size and amount was detected via BioAnalyzer HS Chip (Agilent CA, USA).
Bioanalyzer hs chip
Manufactured by Agilent Technologies
Sourced in United States
The BioAnalyzer HS Chip is a microfluidic device designed for the analysis of nucleic acid samples. It enables the separation, detection, and quantification of DNA, RNA, and other biomolecules in a high-throughput and automated manner.
Automatically generated - may contain errors
Lab products found in correlation
Ion total rna seq kit v2, by Thermo Fisher Scientific (2 mentions)
Magnetic bead cleanup module, by Thermo Fisher Scientific (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Bioanalyzer rna pico kit, by Agilent Technologies (1 mentions)
Ion torrent onetouch system, by Thermo Fisher Scientific (1 mentions)
Ion torrent 316 chip, by Thermo Fisher Scientific (1 mentions)
Ion pgm template ot2 200 kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using bioanalyzer hs chip
1
RNA Isolation and Sequencing Library Preparation
2
Small RNA Sequencing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Small RNA-seq was performed as described previously (27 (link)). In brief, small RNAs were enriched from 2 μg of total RNA using the Magnetic bead cleanup module (Life Technologies). Libraries were then prepared using the Ion Total RNA-Seq Kit v2 (Life Technologies) and analysed via BioAnalyzer HS Chip (Agilent CA, USA). Afterwards, libraries (18 pM each) were clonally amplified by emulsion PCR in the IonTorrent OneTouch System (Life Technologies) using the Ion PGM Template OT2 200 Kit (Life Technologies) according to manufacturer′s protocol. Sequencing was performed in an IonTorrent Personal Genome Machine using an IonTorrent 316 Chip (Life Technologies).
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