The hDPSCs were collected in cold Dulbecco's phosphate-buffered saline (DPBS, Thermo Fisher Scientific) and subsequently incubated in the dark at 4°C for 30 min with FITC-conjugated anti-human CD29, FITC-conjugated anti-human CD44, FITC-conjugated anti-human CD105, FITC-conjugated anti-human CD31, FITC-conjugated anti-human CD34, and FITC-conjugated anti-human CD45 (BD Biosciences, San Jose, CA, USA). The samples were read on a flow cytometer (Beckman Coulter, FC500, FL, USA), and the data were analyzed using FlowJo software (Tree Star, San Carlos, CA, USA).
Fitc conjugated anti human cd29
Manufactured by BD
Sourced in United States
FITC-conjugated anti-human CD29 is a monoclonal antibody that binds to the human CD29 cell surface antigen. CD29 is also known as the β1 integrin subunit and is involved in cell-cell and cell-matrix adhesion.
Automatically generated - may contain errors
Lab products found in correlation
Fitc conjugated anti human cd34, by BD (2 mentions)
Fitc conjugated anti human cd45, by BD (2 mentions)
Fitc conjugated anti human cd44, by BD (2 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions)
Fitc conjugated anti human cd105, by BD (1 mentions)
Fitc conjugated anti human cd31, by BD (1 mentions)
Ifn β, by Thermo Fisher Scientific (1 mentions)
Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions)
Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions)
Recombinant human ifn α, by Thermo Fisher Scientific (1 mentions)
Ifn γ, by Thermo Fisher Scientific (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Recombinant human tgf β, by R&D Systems (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
Il 21, by Thermo Fisher Scientific (1 mentions)
Il 10, by Thermo Fisher Scientific (1 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)
Mouse igg1, by BD (1 mentions)
Mouse igg2a, by BD (1 mentions)
3 protocols using fitc conjugated anti human cd29
1
Immunophenotyping of hDPSCs by Flow Cytometry
2
Immunophenotypic Characterization of Cells
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The following antibodies (to humans) were used in this study: fluorescein isothiocyanate (FITC)‐conjugated antihuman CD29, CD44, HLA‐DR, CD14, phycoerythrin (PE)‐conjugated antihuman CD105, CD166, CD45, CD34, and the respective isotype‐matched control antibodies (mouse IgG1 and mouse IgG2a) (all from BD Biosciences, BD Pharmingen, Fremont, CA, http://www.bdbiosciences.com ). Recombinant human TGF‐β was from R&D Systems and recombinant human IFN‐α, IFN‐β, IFN‐γ, TNF‐α, IL‐6, IL‐1β, IL‐2, IL‐21, IL‐4, and IL‐10 were from PeproTech (Rocky Hill, NJ, https://www.peprotech.com/en-US ).
3
Phenotypic Characterization of hDPSCs
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The hDPSCs were harvested in Dulbecco’s phosphate-buffered saline (DPBS, Thermo Scientific) and incubated for 30 min at 4 °C with FITC-conjugated anti-human CD29, FITC-conjugated anti-human CD44, FITC-conjugated anti-human CD34, and FITC-conjugated anti-human CD45 (BD Biosciences) protected from light. The samples were tested in the flow cytometer (Beckman Coulter, FC500, FL, USA), and the data were analyzed by FlowJo software (Tree Star, San Carlos, CA, USA).
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