Anti cd8 antibody

A Tumor Dissociation Kit, gentleMACS C Tubes, gentleMACS Disssociator, and MACSmix Tube Rotator were obtained from Miltenyi Biotec (Auburn, CA, USA). A Mouse CD3⁺ T Cell Isolation Kit, Mouse CD8⁺ T Cell Isolation Kit, polystyrene round-bottom tube, and Magnet for column-free immunomagnetic separation were obtained from STEMCELL Technologies (Vancouver, BC, Canada). Dynabeads Mouse T-Activator CD3/CD28 was purchased from Life Technologies (Carlsbad, CA, USA). A Cell Counting Kit-8 was purchased from Dojindo Molecular Technologies (Rockville, MD, USA). Oxa was purchased from TargetMol (Wellesley, MA, USA). Mouse IL-2 ELISA was purchased from BD Biosciences (San Diego, CA, USA). Mouse GrB ELISA was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-CD8 antibody and anti-GrB antibody were obtained from Cell Signaling Technology (Danvers, MA, USA).

NHS‐Cy5.5 (Cat. # 47020), NHS‐Sulfo‐Cy5 (Cat. # 43320), MAL‐Sulfo‐Cy5 (Cat. # 43380), NHS‐Cy5 (Cat. # 43020), and MAL‐Cy5 (Cat. # 43080) were purchased from Lumiprobe. d‐Luciferin potassium salt (Cat. # 50227) and doxorubicin (Cat. # D1515) were purchased from Sigma‐Aldrich. SPDP (Cat. # HY‐100216), aldoxorubicin (Cat. # HY‐16261), Pistop 2 (Cat. # HY‐115604), Genistein (Cat. # HY‐14596), and GSH (Cat. # HY‐D0187) were purchased from MedChemExpress. The PD‐10 desalting column was purchased from GE (Cat. # 17085101). HSA and anti‐PD‐L1 antibody were kindly gifts from Zhejiang Hisun Pharmaceutical Co., Ltd. Anti‐CD8 antibody was purchased from Cell Signaling Technology (Cat. # 98941S). RBD protein was expressed and purified from Pichia pastoris by our collaborators.³⁵ All mice (BALB/c nude and C57BL/6) were purchased from Charles River.