Rabbit anti phospho jak1

Manufactured by Cell Signaling Technology

Rabbit anti-phospho-JAK1 is an antibody that recognizes the phosphorylated form of the JAK1 protein. JAK1 is a non-receptor tyrosine kinase that plays a crucial role in signal transduction pathways.

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Lab products found in correlation

Anti tuj1, by BioLegend (1 mentions) Rabbit anti gfp a11122, by Thermo Fisher Scientific (1 mentions) Alpha tubulin dm1a, by Cell Signaling Technology (1 mentions) Mouse anti ha 11, by BioLegend (1 mentions) Rabbit anti myc, by Cell Signaling Technology (1 mentions) Rabbit anti stat3, by Cell Signaling Technology (1 mentions) Rabbit anti phospho stat3, by Cell Signaling Technology (1 mentions) Mouse anti neun mab377, by Merck Group (1 mentions) Alexa fluor 555 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Rabbit anti jak1, by Santa Cruz Biotechnology (1 mentions) Rabbit anti v5, by Cell Signaling Technology (1 mentions) Mouse anti tubulin, by Merck Group (1 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Mouse anti flag, by Merck Group (1 mentions)

2 protocols using rabbit anti phospho jak1

Immunoblotting Antibody Reagents

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The following antibodies, from the indicated sources, were used: rabbit anti-phospho-STAT3 (#9145), rabbit anti-STAT3 (#8768), rabbit anti-phospho-JAK1 (#74129), rabbit anti-myc (#9145), alpha-tubulin (DM1A, #3873) all from Cell Signaling Technology; mouse anti HA11 (#901514) and anti-Tuj1 (both from Biolegend); mouse anti-myc 9E10 (UPenn Cell Center); rabbit anti-GFP #A11122 (Invitrogen); mouse anti-JAK1 #610231 (BD Transduction Labs), mouse-anti NeuN #MAB377 (Millipore Sigma), Calnexin #10286 (Abcam). Unless otherwise noted, all chemicals were from ThermoFisher Biosciences and were of the highest reagent grade available.

Hernandez L.M., Montersino A., Niu J., Guo S., Faezov B., Sanders S.S., Dunbrack R.L, & Thomas G.M. (2023). Palmitoylation-dependent control of JAK1 kinase signaling governs responses to neuropoietic cytokines and survival in DRG neurons. The Journal of Biological Chemistry, 299(8), 104965.

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Western Blot Analysis of Immune Signaling Proteins

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The following primary antibodies were used: Mouse anti-FLAG (Sigma, F3165, 1:1,000), Mouse anti-tubulin (Sigma, T9026, 1:10,000), Rabbit anti-STAT1 or 2 (Cell Signaling, 9127, 72604, 1:1,000), Rabbit anti-phospho-STAT1 or 2 (Cell Signaling, 88410, 9167, 1:1,000), Rabbit anti-V5 (Cell Signaling, 13202, 1:1,000), Rabbit anti-JAK1 (Santa-Cruz Biotechnology, sc-277; 1:400), Rabbit anti-phospho-JAK1 (Cell Signaling, 74129, 1:1000), Rabbit anti-lamin (Abcam, 1:1000). The secondary antibodies used were HRP anti-mouse and anti-rabbit (Jackson Immuno Research, 1:5000), Alexa fluor 488 goat anti-rabbit (Invitrogen, A11034, 1:500) or Alexa fluor 555 goat anti-rabbit (Invitrogen, A27039, 1:500).

Moalem Y., Malis Y., Voloshin K., Dukhovny A., Hirschberg K, & Sklan E.H. (2022). Sandfly Fever Viruses Attenuate the Type I Interferon Response by Targeting the Phosphorylation of JAK-STAT Components. Frontiers in Immunology, 13, 865797.

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