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Af647 conjugated human fibrinogen

Manufactured by Thermo Fisher Scientific
Sourced in United States

AF647 conjugated human fibrinogen is a fluorescently labeled version of the human blood clotting protein fibrinogen. Fibrinogen is a key component in the process of blood clot formation. The AF647 fluorescent dye is covalently attached to the fibrinogen molecule, allowing it to be detected and visualized in various biological applications.

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2 protocols using af647 conjugated human fibrinogen

1

Multicolor Flow Cytometry of Platelet Activation

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Reagents were obtained as follows: Alexa Fluor (AF) 647 conjugated anti-human CD41 (Bio-Rad, Raleigh, NC, USA), Anti-human CD18 antibody (clone TS1/18), PE anti-human CD11a (BioLegend, San Diego, CA, USA), AF647 conjugated human fibrinogen (ThermoFisher Scientific, Grand Island, NY, USA), Sytox-green (Life Technologies, Grand Island, NY, USA), fibrillar collagen (type I, Chrono-log, PA, USA), Dade Innovin lipidated tissue factor (TF, Siemens, Malvern, PA, USA), D-Phe-Pro-Arg-CMK (PPACK, Santa Cruz Biotechnology, Dallas, TX, USA), corn trypsin inhibitor (CTI, Haematologic Technologies, Essex Junction, VT, USA), tPA (Abcam, Cambridge, MA, USA), H-Gly-Pro-Arg-Pro-OH acetate salt (GPRP, Bachem Americas, Torrance, CA, USA), ε-aminocaproic acid (εACA), ethylenediaminetetraacetic acid (EDTA), and Sigmacote (Sigma, St. Louis, MO, USA).
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2

Evaluating Platelet Function and Coagulation

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Reagents were obtained as follows: Alexa Fluor 488 conjugated anti‐human CD61 (Bio‐Rad), AF647 conjugated human fibrinogen (ThermoFisher Scientific), type I fibrillar collagen (Chrono‐log), Dade Innovin lipidated tissue factor (TF, Siemens), CTI and FXIa (Haematologic Technologies), 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES; Fisher Scientific), Sigmacote (Sigma), Anti‐FVIII (4A4) and anti‐FIX (BC2; Green Mountain Antibodies), Anti‐TFPI (gA200 hIgG1, Bayer, Whippany), bispecific antibody (emicizumab), reFIX‐V181T variant,25 and a recombinant rIgG4 containing complementarity‐determining regions originally derived from a patient (NB41, as described in van den Brink26).
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