Inos antibodies

Astilbin (purity > 98%) was purchased from BOC Sciences (New York, USA). Cell Counting Kit‐8 (CCK‐8) was purchased from Dojindo (Kumamoto, Japan). LPS, type II collagenase and dimethylsulphoxide (DMSO) were obtained from Sigma‐Aldrich (St Louis, MO, USA). The primary antibodies against TLR4, MyD88, TRAF6, Lamin B1 and GADPH were obtained from Abcam (Cambridge, UK); iNOS antibodies were obtained from Sigma‐Aldrich (St Louis, MO, USA); and MD2 antibody was purchased from eBioscience (eBioscience, SanDiego, CA). Antibodies against COX‐2, p65 and IκBα were purchased from Cell Signaling Technology (Danvers, MA, USA); anti‐IRAK1, goat anti‐rabbit and antimouse IgG‐HRP were from Bioworld (OH, USA). Recombinant human MD2 and TLR4 protein (rhMD2 and rhTLR4) were purchased from R&D Systems (Minneapolis, MN, USA). Biotin‐labelled LPS (Biotin‐LPS) was obtained from InvivoGen (San Diego, CA, USA). Alexa Fluor^®488 labelled and Alexa Fluor^®594 labelled goat anti‐rabbit IgG (H + L) the second antibody were purchased from Jackson ImmunoResearch (West Grove, PA, USA). 4′,6‐Diamidino‐2‐phenylindole (DAPI) was purchased from Beyotime (Shanghai, China), and cell culture reagents were obtained from Gibco (Grand Island, NY, USA).

Arctigenin (purity > 98%) was purchased from the Tianjin Shilan Technology Company. Cell culture reagents were purchased from Gibco (Life Technologies Corp.). Cell Counting Kit‐8 (CCK‐8) was bought from DOJINDO (Kumano, Japan). Recombinant human IL‐1β protein was supplied by Novoprotein. Type II collagenase, sodium carboxymethyl cellulose (CMC‐Na) and dimethyl sulphoxide (DMSO) were received from Solarbio. The primary antibody against Lamin B and GAPDH was obtained from Abcam, iNOS antibodies were obtained from Sigma‐Aldrich, and goat anti‐rabbit and antimouse IgG‐HRP were from Bioworld (OH). The anti‐COX‐2, anti‐p65, anti‐IκBα, anti‐PI3K, anti‐p‐PI3K, anti‐Akt and anti‐p‐Akt antibodies were acquired from Abcam. The second antibody (Alexa Fluor® 488‐labelled goat anti‐rabbit IgG) was achieved from Yeasen. The 4', 6‐diamidino‐2‐phenylindole (DAPI) was purchased from Beyotime.