The pGC apoptosis rate was determined using the Annexin V FITC/Propidium Iodide Kit (Vazyme Biotech, China), according to the manufacturer’s instructions. Cells were sorted by fluorescence-activated cell sorting using a Beckman Coulter instrument (Becton Dickinson, Franklin, NJ, USA).
Annexin 5 fitc propidium iodide kit
Manufactured by Vazyme
Sourced in China
The Annexin V FITC/Propidium Iodide Kit is a lab equipment product that can be used to detect apoptosis and cell viability. The kit contains Annexin V conjugated with the fluorescent dye FITC and Propidium Iodide. These reagents are used to stain cells and can be analyzed using flow cytometry or fluorescence microscopy.
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Lab products found in correlation
2 protocols using annexin 5 fitc propidium iodide kit
1
Apoptosis Rate Determination by Flow Cytometry
2
Annexin V-FITC/PI Apoptosis Assay
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Apoptotic cells were detected using the Annexin V-FITC/propidium iodide kit (Vazyme Biotech, Nanjing, China) according to the manufacturer’s instructions. In brief, the palmitate-treated cells were washed twice with PBS and dislodged using 0.25% trypsin. Cells were centrifuged and resuspended in PBS containing 50 µg/mL propidium iodide, 0.1 g/L RNase, and 1% bovine serum albumin. The cells were then incubated at 37°C for 30 min in the dark before analysis.
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