Antibodies specific to β actin

HSV1(F) (provided by B. Roizman, University of Chicago, USA) was used for infection experiments. The strain HSV1(17+)lox (provided by B. Sodeik, Hannover Medical School, Germany) was used as PCR template. HSV1 propagation and virus growth curves were performed as described [14 (link)]. To monitor infection, Vero cells were infected with HSV1(F) at the indicated MOI. Cell lysates were prepared at the indicated times post infection and analysed by Western blotting using primary antibodies to the immediate early proteins ICP0 (anti-ICP0, Santa Cruz) and ICP27 (anti-ICP27, Virusys), to the early protein gB (anti-Glykoprotein B, Santa Cruz) and to the late proteins VP5 (anti-ICP5 (VP5), Abcam) and pUL34 [9 (link)] followed by secondary antibodies conjugated to POX. Antibodies specific to β-actin (Abcam) were used as control.

Human GBM cell lines U251 and U87 were purchased from the Shanghai Cell Bank, Chinese Academy of Sciences for this study. These cell lines were cultured in DMEM supplemented with 10% FBS at 37°C in a humidified incubator with 5% CO₂. Antibodies against MALT1, p‐pRb, cyclin D1, CDK4, p27, p21, p65, IκB‐α and lamin A/C were obtained from Cell Signaling Technology (CST, Beverly, MA, USA). Antibodies specific to β‐actin were purchased from Abcam (Cambridge, MA, USA). DAPI was obtained from Sigma‐Aldrich Chemical Co. (St. Louis, MO, USA).