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24 well milli cell hanging insert

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The 24-well milli-cell hanging insert is a laboratory equipment designed for cellular culture applications. It provides a platform for culturing cells in a suspended manner within a multiwell plate format.

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Lab products found in correlation

24 well biocoat matrigel invasion chambers, by BD (2 mentions) Spot imaging software, by Nikon (2 mentions)

Close spelling variants of this product

24-well insert (22 citations)

2 protocols using 24 well milli cell hanging insert

1

Cell Migration and Invasion Assay

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Migration and invasion assays were performed in 24-well milli-cell hanging insert (BD Biosciences) or 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences). In brief, 5 × 104 cells were seeded to the top chamber and 10% FBS in medium was added to the bottom chamber as a chemoattractant. After 24 or 48 h incubation, the number of cells that invaded through the membrane (migration) or Matrigel (invasion) was counted in 10 fields and imaged using SPOT imaging software (Nikon).
Jia Y., Yan Q., Zheng Y., Li L., Zhang B., Chang Z., Wang Z., Tang H., Qin Y, & Guan X.Y. (2022). Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer. Journal of Experimental & Clinical Cancer Research : CR, 41, 287.
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2

Cell Migration and Invasion Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Migration and invasion assays were performed in 24-well milli-cell hanging insert (BD Biosciences) or 24well BioCoat Matrigel Invasion Chambers (BD Biosciences). In brief, 5×10 4 cells were seeded to the top chamber and 10% FBS in medium was added to the bottom chamber as a chemoattractant. After 24 or 48h incubation, the number of cells that invaded through the membrane (migration) or Matrigel (invasion) was counted in 10 fields and imaged using SPOT imaging software (Nikon).
Jia Y., Yan Q., Zheng Y., Li L., Zhang B., Tang H., Qin Y., & Guan X. (2022). Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer.
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