Anti mouse immunoglobulin g and anti rabbit immunoglobulin g fluorescent conjugated secondary antibodies

GSK126 (Figure 1A) was purchased from Shanghai Hope Chem Co., Ltd. (Shanghai, China). MG132 was from EMD Millipore Corporation (Billerica, MA). z-DEVD-fmk and bortezomib were from Selleck (Shanghai, China). Antibodies against H3, AIF, BCL-X_L, MCL-1 (S-19), BIM, GSK3β, BAX, DVL3, Survivin and c-MYC were from Santa Cruz Biotech (Santa Cruz, CA). Antibodies against EZH2, PARP (clone 4C10-5), active-caspase-3, cytochrome c (clone 6H2.B4), XIAP, BCL-2, phospho-GSK3β (Y216), β-catenin were purchased from BD Biosciences (San Jose, CA). Antibodies against H3K27me3, caspase-3, caspase-8, caspase-9, DVL2, and phospho-GSK3β (S9) were from Cell Signaling Technology (Danvers, MA). Antibody against cytochrome c oxidase subunit II (COX II) was from Molecular Probes (Eugene, OR). Antibodies against Axin2 and LEF1 were from Sigma-Aldrich (Shanghai, China). Anti-mouse immunoglobulin G and anti-rabbit immunoglobulin G fluorescent-conjugated secondary antibodies were from LI-COR Biotechnology (Nebraska).

Tenovin-6 (chemical structure, Fig. 1A) was purchased from Cayman Chemical (Ann Arbor, MI). Annexin V–FITC and N-acetylcysteine (NAC) were from Sigma-Aldrich (Shanghai, China). CM-H2DCF-DA and antibody against cytochrome c oxidase subunit II (COXII) were purchased from Invitrogen (Shanghai, China). Antibodies against SIRT1 (H-300), p53 (DO-7), Bax, DVL1, DVL3, β-catenin, cyclin D1 (C-20), survivin and c-Myc were from Santa Cruz Biotech. (Santa Cruz, CA). Antibody against active-β-catenin was from Millipore and the fluorescent secondary antibody was purchased from life technologies (Eugene. OR). Antibodies against PARP (clone 4C10-5), caspase-3, cytochrome c (clone 6H2.B4), XIAP, Bcl-2, phospho-GSK3β (Y216) were purchased from BD Biosciences (San Jose, CA). Antibodies against K382-acetyl-p53, DVL2, phospho-GSK3β (S9), MMP2 and MMP9 were from Cell Signaling Tech. (Beverly, MA). Anti-SIRT2 was purchased from Atlas Antibodies. Anti-mouse immunoglobulin G and anti-rabbit immunoglobulin G fluorescent-conjugated secondary antibodies were from LI-COR Biotechnology (Nebraska, USA).