The commercially available ELISA procedure (Human SAA KHA0011C, Invitrogen, California, USA) was used to measure SAA concentration in serum, HDL2, and HDL3, specifically detecting SAA1. As per the manufacturer's instructions, analysis was performed on a TRITURUS ELISA system (Grifols, Vicopisano, Italy). Prior to analyses, samples were diluted as follows: serum, 1 : 150; HDL2, 1 : 10; and HDL3, 1 : 100. The CVs for SAA were 2.8%, 3.6%, and 5.6% (interspecific) and 8.0%, 9.5%, and 11% (intraspecific) for SAA, HDL2, and HDL3, respectively.
Human saa kha0011c
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Human SAA KHA0011C is a laboratory equipment product. It is designed to measure levels of the Serum Amyloid A (SAA) protein in human samples. The core function of this product is to provide quantitative analysis of SAA concentrations.
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Lab products found in correlation
2 protocols using human saa kha0011c
1
Quantifying Serum SAA Levels
2
Quantifying Serum and HDL-Associated SAA
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The concentration of SAA was measured in serum, HDL2 and HDL3 by a commercially available ELISA procedure, which measures SAA-1 (Invitrogen, Human SAA KHA0011C). SAA was assessed on a Grifols TRITURUS automated ELISA system (Italy), as per the manufacturer's instructions, with the following modifications: Serum was diluted 1:150, HDL2 was diluted 1:10 and HDL3 diluted 1:100 prior to analysis. The intra-assay CVs for serum-, HDL2-and HDL3-SAA were 2.8, 3.7 and 5.6%, respectively, while their inter-assay CVs were 8, 9.5 and 11%, respectively. Non-protein standardised results are presented as µg/L, which is the absolute concentration in the serum or in the HDL2 and HDL3 subfractions after isolation. In addition, SAA associated with HDL2 and HDL3 was also standardised for protein concentration and is presented as μg SAA/mg protein.
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