α glycerophosphate dehydrogenase triosephosphate isomerase

N-Isopropylacrylamide (NIPAm) (Sigma-Aldrich, 99%) was recrystallized from a mixture of n-hexane/toluene (4 : 1 v/v). N-2-thiolactoneacrylamide (TlaAm) was synthesized according to a procedure described in a previous publication.^{8 (link)}N,N′-methylenebisacrylamide (Bis) (Sigma-Aldrich, 99%), N,N,N′,N′-tetramethylethylenediamine (TEMED) (Carl Roth, ≥99%), potassium persulfate (Sigma-Aldrich, ≥99.0%), nicotinamide adenine dinucleotide disodium salt (NADH) (Roche, ∼100%, grade I), 2-deoxy-d-ribose-5-phosphate sodium salt (DRP) (Sigma, ≥95%), glucose (Sigma-Aldrich, ≥99.5%), dimethyl sulfoxide (DMSO) (Carl Roth, ≥99.8%, <100 ppm H₂O), deuterated chloroform (Carl Roth, 99.8% D), Atto-665-maleimide (Atto-Tec), bovine serum albumin (BSA) (Sigma-Aldrich, >98%, lyophilized powder, heat shock fraction), peroxidase from horseradish (HRP) (Sigma-Aldrich), (lyophilized powder, 150 U mg⁻¹), glucose oxidase from Aspergillus niger (Carl Roth, lyophilized powder, 280 U mg⁻¹) and α-glycerophosphate dehydrogenase-triosephosphate isomerase from rabbit muscle (Sigma-Aldrich), Type III, ammonium sulfate suspension, TPI 750–2000 units per mg protein, GDH 75–200 units per mg protein (biuret) were used as received.