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Waters uplc esi q tof ms system

Manufactured by Waters Corporation

The Waters UPLC-ESI-Q/TOF-MS system is a high-performance liquid chromatography (HPLC) coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q/TOF-MS). The system is designed to perform high-resolution, accurate-mass analysis of a wide range of samples.

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2 protocols using waters uplc esi q tof ms system

1

Urine Sample Preparation for UPLC-MS

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Urine samples were prepared by mixing 50 μL of urine with 150 μL of 50% (v/v) aqueous methanol. After 20,000 g centrifugation at 4°C for 10 min, a 10 μL aliquot of the supernatants was injected into a Waters UPLC-ESI-Q/TOF-MS system (Waters Corporation, Milford, MA) for analysis.
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2

Serum Metabolite Extraction and UPLC-MS Analysis

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Serum samples were prepared through mixing 10 μL serum with 190 μL 66% aqueous acetonitrile containing 5 μM chlorpropamide. After 14,000×g centrifugation for 20 min, a 5 μL aliquot of the supernatants was injected into a Waters UPLC-ESI-QTOFMS system (Waters Corporation, Milford, MA) for analysis. Separation of serum components was performed using an Acquity UPLC BEH C18 column (1.7 μm, 2.1 × 50 mm, Waters Corp.). The mobile phase was comprised of 0.1% aqueous formic acid (A) and acetonitrile containing 0.1% formic acid (B). The running method was described in a previous publication (Fang et al. 2013 (link)).
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