Rat anti mouse galectin 9 clone 108a2

Manufactured by BioLegend

The Rat anti-mouse galectin-9 clone 108A2 is a laboratory reagent used to detect the presence of galectin-9 in mouse samples. Galectin-9 is a protein involved in various biological processes. This antibody can be used in techniques such as flow cytometry and immunohistochemistry to identify and quantify galectin-9 expression.

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Lab products found in correlation

Mouse anti β actin clone ac 74, by Merck Group (1 mentions) Odyssey blocking buffer obb, by LI COR (1 mentions) Anti β actin clone ac 15, by Santa Cruz Biotechnology (1 mentions) Irdye 680lt goat anti rabbit igg, by LI COR (1 mentions) Alexa fluor 488 conjugated anti rat igg, by Thermo Fisher Scientific (1 mentions) Irdye 680lt donkey anti rabbit igg, by LI COR (1 mentions) Alexa fluor 488 conjugated anti goat igg, by Thermo Fisher Scientific (1 mentions) Irdye 680lt goat anti mouse igg, by LI COR (1 mentions) Irdye 800cw donkey anti goat igg, by LI COR (1 mentions) Alexa fluor 568 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions)

2 protocols using rat anti mouse galectin 9 clone 108a2

Galectin Antibody Immunoassay Protocol

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Experiments were performed as previously described in Lhuillier et al.^{31 (link)}. The following primary antibodies were used: polyclonal goat anti-mouse gal-1 (R&D Systems Cat# AF1245, RRID:AB_354693), mouse anti-human/murine gal-3 clone B2C10 (Santa Cruz Biotechnology Cat# sc-32790, RRID:AB_627657), polyclonal rabbit anti-human/murine gal-8 (#MBS828618, MyBioSource), rat anti-mouse galectin-9 clone 108A2 (BioLegend Cat# 137,901, RRID:AB_10568691); mouse anti-β-actin clone AC-74 (Sigma-Aldrich Cat# A2228, RRID:AB_476697).

Baloche V., Rivière J., Tran T.B., Gelin A., Bawa O., Signolle N., Diop M.K., Dessen P., Beq S., David M, & Busson P. (2021). Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model. Scientific Reports, 11, 5227.

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Versatile Immunoblotting Reagents for Galectin Detection

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Specific reagents included: Odyssey® blocking buffer (OBB) from Li-Cor Biosciences (Lincoln, NE); goat anti-mouse galectin-1 from R&D Systems (Minneapolis, MN); a hybridoma secreting rat anti-human/mouse galectin-3 (mAb M3/38) from ATCC (Manassas, VA); rabbit anti-mouse galectin-7 from Bethyl Laboratories (Montgomery, TX); rabbit anti-human/mouse galectin-8 from Novus Biologicals (Littleton, CO); rat anti-mouse galectin-9 (clone 108A2) from BioLegend (San Diego, CA); anti-β-actin (clone AC-15) from Santa Cruz Biotechnology (Dallas, TX). Infrared secondary antibodies obtained from Li-Cor were: donkey anti-goat IgG IRDye 800CW, donkey anti-rabbit IgG IRDye 680LT, goat anti-rat IgG IRDye 800CW, goat anti-rabbit IgG IRDye 680LT, and goat anti-mouse IgG IRDye 680LT. Fluorescence-labeled secondary antibodies purchased from Life technologies (Grand Island, NY) were: Alexa Fluor® 488-conjugated anti-rat IgG, Alexa Fluor 568®-conjugated anti-rabbit IgG, and Alexa Fluor 488®-conjugated anti-goat IgG.

Sugaya S., Chen W.S., Cao Z., Kenyon K.R., Yamaguchi T., Omoto M., Hamrah P, & Panjwani N. (2015). Comparison of galectin expression signatures in rejected and accepted murine corneal allografts. Cornea, 34(6), 675-681.

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