Mouse anti histone h2b

The exosome pellets in ultracentrifugation tubes were lysed by the direct addition of RIPA buffer. After lysis, the protein concentration was quantified, and then we prepared western blotting samples with 20 μg of exosome protein. To detect proteins, the lysates were subjected to SDS-PAGE on 10 % acrylamide gels, with electrophoretic transfer to nitrocellulose membranes. The membranes were incubated with primary antibodies (1 : 1000) and secondary antibodies (1 : 5000), and proteins were visualized using an ECL kit (Elpis Biotech, South Korea). The following antibodies were used: mouse anti-TSG101 antibody (Abcam, UK), rabbit anti-CD81 (Abcam, UK), mouse anti-histone H2B (Abcam, UK), and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (heavy and light chain [H/L]; Invitrogen, USA) and goat anti-rabbit IgG (H/L; Abcam, UK).

Rabbit-anti-ID3 (Cell Signaling, Cat#9837) and for IP of endogenous ID3 we used agarose beads conjugated with mouse-anti-ID3(Santa Cruz, Cat#sc-56712). Mouse-anti-beta-Actin (Santa Cruz, Cat#sc-47778). Mouse-anti-Vinculin (Santa Cruz, Cat#sc-25336). Mouse-anti-phospho-Histone H2A.X (S139) (Merck, Cat#05-636). Rabbit-anti-phospho-Histone H2A.X (S139) (Abcam, Cat# ab2893). Rabbit-anti-RAD51 (Calbiochem, Cat#PC130). Rabbit-anti-RAD51 (Abcam, Cat#ab176458). Rabbit-anti-XRCC4 (GenTex, Cat#GTX109632). Rabbit-anti-phospho-RPA (S4/S8) (Bethyl, Cat#A300-245A). Mouse-anti-Histone H2B (Abcam, Cat#ab52484). Rabbit-anti-DNA-PKsc (Cell Signaling, Cat#4602). Rabbit-anti-CtIP (Abcam, Cat#70163). Goat-anti-MDC1 (Santa Cruz, Cat#sc-27737). Rabbit-anti-MDC1 (Abcam, Cat#ab11169). Rabbit-anti-NBS1 (Novus Biologicals, Cat#NB100-143). Mouse-anti-RAD50 (Abcam, Cat#ab89). Rabbit-anti-RECQL (Abcam, Cat#ab151501). Mouse-anti-RPA32 (Santa Cruz, Cat#sc-53496). Mouse-anti-Flag-HRP (Sigma, Cat#A8592). Mouse-anti-IgG (Santa Cruz, Cat#sc-2025). Mouse-anti-CenpF (BD bioscience, Cat#610768). Goat-anti-mouse IgG-HRP (Cell Signaling, Cat#7076P2). Goat-anti-rabbit IgG-HRP (Cell Signaling, Cat#7074S). Goat-anti-mouse IgG-AlexaFluor 594 (Molecular Probes, Cat#A11005). Goat-anti-rabbit IgG-AlexaFluor 488 (Molecular Probes, Cat#A11008).