Gtx128092

Manufactured by GeneTex

GTX128092 is a high-performance laboratory centrifuge designed for efficient separation of samples. It features a robust stainless steel construction, digital speed and time controls, and a range of rotor options to accommodate various sample volumes and tube sizes.

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Lab products found in correlation

Gtx127277, by GeneTex (1 mentions) Gtx70220, by GeneTex (1 mentions) Recombinant human furin, by R&D Systems (1 mentions) Nigericin, by Merck Group (1 mentions) Dansylsarcosine piperidinium salt, by Merck Group (1 mentions) Antibodies against, by Cell Signaling Technology (1 mentions) Anti mouse igg dylight 488, by Abbkine (1 mentions) Human il 1β elisa kit, by BD (1 mentions) Anti β actin antibody 66009, by Proteintech (1 mentions) Anti mouse igg dylight 649, by Abbkine (1 mentions) Anti rabbit igg dylight 649, by Abbkine (1 mentions) D4d8t, by Cell Signaling Technology (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions)

3 protocols using gtx128092

Immunoblot Analysis of DENV Infection

Cited 1 time

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Immunoblot assays were performed as previously described (Marceau et al., 2016 (link)). HEK293 cells were infected with DENV (MOI=0.5) and treated with different concentrations of NGI-1 for 52h. As control, cells were treated with 10μg/ml tunicamycin for 4h before harvest. To detect DENV proteins, anti-prM (Genetex GTX128092) at a dilution of 1:1000, anti-E (Genetex GTX127277) at a dilution of 1:2000, and anti-NS1 (Genetex GTX630556) at a dilution of 1:2000 were used. As loading control anti-p84 (Genetex GTX70220) at a dilution of 1:5000 was used.

Puschnik A.S., Marceau C.D., Ooi Y.S., Majzoub K., Rinis N., Contessa J.N, & Carette J.E. (2017). A small molecule oligosaccharyltransferase inhibitor with pan-flaviviral activity. Cell reports, 21(11), 3032-3039.

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Evaluating Furin Expression in Cells

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Furin expression in cells was evaluated by Western blotting. Briefly, monolayers of Vero and Vero-furin cells were lysed in 1% Triton, 100 mM Tris, 2 M NaCl, and 100 mM EDTA; lysates were then clarified by centrifugation at 3,500 x g. Recombinant human furin (R&D Systems) was loaded in gels as a reference. Furin was detected using primate and human furin-reactive antibody (ThermoFisher) at 2 ug/ml. prM and E proteins in partially purified virus preparations were detected using monoclonal antibodies (mAbs) GTX128092 (Genetex) and, 4G2 respectively, at 1 μg/ml.

Mukherjee S., Sirohi D., Dowd K.A., Chen Z., Diamond M.S., Kuhn R.J, & Pierson T.C. (2016). Enhancing dengue virus maturation using a stable furin over-expressing cell line. Virology, 497, 33-40.

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Inflammasome Activation and Cytokine Release

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Phorbol-12-myristate-13-acetate (PMA), LPS, nigericin, ATP, and dansylsarcosine piperidinium salt (DSS) were purchased from Sigma-Aldrich. Caspase-1 inhibitor (VX-765) was purchased from Selleck. Recombinant human IL-1β protein (200-01B) and human IL-1RA (200-01RA) were obtained from Peprotech. Recombinant mouse IL-1β protein (401-ML/CF) and recombinant mouse IL-1RA (480-RM/CF) were purchased from R&D Systems. Trizol reagent was from Invitrogen, and Lipofectamine 2000 was from Invitrogen. The human IL-1β ELISA kit was purchased from BD Biosciences, the mouse IL-1β and tumor necrosis factor (TNF)-α ELISA kits were purchased from R&D Systems, and the LPS ELISA kit was purchased from Expandbio (Beijing, China).
Antibodies against NLRP3 (D4d8T), caspase-1 (D7F10), and IL-1β (D3U3E and 3A6) were purchased from Cell Signaling Technology. Antibody against DENV-NS3 (GTX124252) and DENV-Prm (GTX128092) were purchased from Genetex. Anti-β-actin antibody (66009) was purchased from Proteintech. Antibody against ASC (sc-271054) was purchased from Santa Cruz Biotechnology. Anti-mouse IgG Dylight 649, anti-mouse IgG Dylight 488, anti-rabbit IgG Dylight 649, and anti-rabbit IgG fluorescein isothiocyanate (FITC) were purchased from Abbkine.

Pan P., Zhang Q., Liu W., Wang W., Yu Z., Lao Z., Zhang W., Shen M., Wan P., Xiao F., Shereen M.A., Zhang W., Tan Q., Liu Y., Liu X., Wu K., Liu Y., Li G, & Wu J. (2019). Dengue Virus Infection Activates Interleukin-1β to Induce Tissue Injury and Vascular Leakage. Frontiers in Microbiology, 10, 2637.

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