A cell counting kit-8 (CCK8) (Shanghai Beyotime Biotechnology Co., Ltd. (China)) was applied to detect the cell proliferation ability of ME-180 and C33A cells. The cells were transferred to a 96-well plate at 5×103 cells/well, and CCK-8 solution (10 μL) was added into each detection well at 24, 48, 72, and 96 hrs after culturing. After each addition of the solution, the plate was continuously incubated for 2 hrs. Subsequently, the OD of each well at 450 nm was measured by a Multiskan FC enzyme mark instrument (Thermo Fisher Scientific, Shanghai, China). The assay was repeated three times.
8 cck8
Manufactured by Beyotime
Sourced in China
The -8 (CCK8) is a laboratory equipment product used for various applications. It serves as a core function in assessing cell viability and cytotoxicity. The product's technical specifications and performance details are available upon request.
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Lab products found in correlation
2 protocols using 8 cck8
1
Cell Proliferation Assay for ME-180 and C33A
2
Cell Viability Assay with Trastuzumab
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Cells were seeded in 96-well plates at a density of 5.0 × 103 cells per well, cultured for 24 h, and treated with trastuzumab (Roche Ltd., Basel, Switzerland) at various concentrations for 48 h or the indicated durations. The cells were then incubated with 10 μL cell counting kit-8 (CCK-8) (Beyotime Biotechnology LLC, Shanghai, China) solution for 30‒45 min at 37 °C. Absorbance at 450 nm was detected using a microplate reader (Bio-Rad Model 680). The cell inhibitory index was calculated as [1-(A450 sample-A450 blank) / (A450 control-A450 blank)] × 100%.
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