Ingenia 3.0 t system

Scans were acquired on an Ingenia 3.0-T system (Philips Healthcare, Best, The Netherlands) combined with a commercially available small animal coil (Philips Research, Hamburg, Germany) with heating function to preserve body temperature during the examination. The protocol consisted of one axial multi-shot T2-weighted turbo-spin-echo (TSE) sequence with the following parameters: echo time 65 ms, repetition time 1,540 ms, flip angle 90°, field of view 40 × 40 mm, matrix 256 × 256, slice thickness 1 mm without gap, acquired voxel size 0.19 × 0.27 × 1 mm, and reconstructed voxel size 0.15 × 0.15 × 1 mm, number of signal acquisitions 6. Standard scan time for 15 slices was 5:05 min. The detailed scan protocol of the T2-weighted sequence is available at the supplement for all vendors, as well as an ExamCard for Philips 3.0T Achieva and Ingenia MRI systems ready to use.

MR images were acquired on an Achieva 1.5 T, Achieva 3.0 T system, or Ingenia 3.0 T system (all from Philips Healthcare; Best, the Netherlands). Imaging sequences included a sagittal and coronal T2-weighted fast spin–echo sequence (section thickness, 3.0 mm; slice gap, 0.3 mm; field of view [FOV], 160 mm; TE, 80–90 ms; TR, 3000 ms; flip angle, 90°). Dynamic MRI images were acquired using a sagittal and coronal T1-weighted fast spin–echo sequence (section thickness, 3.0 mm; slice gap, 0.3 mm; FOV, 160 mm; TE, 12 ms; TR, 500–600 ms; flip angle, 80–90°). A bolus injection of 0.1 mmol/kg of the contrast agent (gadoteridol; ProHance; Bracco Eisai, Tokyo) was administered at 2.5 ml/s. A dynamic MRI scan was performed after contrast injection with 15 scans at 11-, 12-, or 13.2-s intervals.

All participants were scanned with a Philips Ingenia 3.0-T system at the Laboratorio Clínico, Centro Integral de Diagnóstico Médico of Guadalajara’s Grupo Río Center (Jalisco, México). A T1-weighted turbo field echo (TFE) structural image was obtained for each subject with a 3-dimensional protocol (repetition time [TR] = 2.8 ms, echo time [TE] = 6.3 ms, 170 slices, and field of view [FOV] = 240 × 240 × 170). The scan time of the T1 had a duration of 2 minutes and 52 seconds per participant. The image acquisition was in the sagittal plane. For the functional images, a T2*-weighted (BOLD) image was obtained (TR = 2 ms, TE = 30 ms, FOV = 230 × 230 × 160, voxel size = 2.4 × 2.4 × 4 mm, 29 slices). The image acquisition was in the transverse plane. The resting-state scanning had a duration of 10 minutes. During scanning, the participants were instructed to relax, remain awake, and keep their eyes open and fixed on a cross symbol on the screen.