Human bm mscs

Manufactured by American Type Culture Collection

Sourced in United States

Human BM-MSCs are primary mesenchymal stem cells derived from human bone marrow. They are characterized by their ability to differentiate into various cell types, including osteoblasts, chondrocytes, and adipocytes. The cells are cryopreserved and available for research and development purposes.

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Lab products found in correlation

Ab10401, by Abcam (1 mentions) Dmem low glucose, by Fujifilm (1 mentions) Be 2 c, by American Type Culture Collection (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Mhcc97h, by American Type Culture Collection (1 mentions) Hl 7702, by American Type Culture Collection (1 mentions) Hcclm3, by American Type Culture Collection (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) 175 cm2 cell culture flasks, by Corning (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

BM-MSCs (13 citations) Human MSCs (2 citations) Healthy human primary BM‐MSCs (2 citations)

4 protocols using human bm mscs

Neuroblastoma and BM-MSCs Culture

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The neuroblastoma cell line BE(2)‐C was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured in DMEM and Ham's F‐12 (DMEM/F‐12; Wako, Osaka, Japan) supplemented with 10% FBS. We purchased human BM‐MSCs from the ATCC and maintained them in low‐glucose DMEM (Wako) supplemented with 10% FBS.
Recombinant human CXCL1 and CXCL2 were obtained from R&D Systems (Minneapolis, MN, USA; 275‐GR and 276‐GB). A CXCR2‐specific antibody for neutralization was purchased from Abcam (ab10401; Cambridge, MA, USA).

Hashimoto O., Yoshida M., Koma Y., Yanai T., Hasegawa D., Kosaka Y., Nishimura N, & Yokozaki H. (2016). Collaboration of cancer‐associated fibroblasts and tumour‐associated macrophages for neuroblastoma development. The Journal of Pathology, 240(2), 211-223.

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3D Bioprinting of BM-MSCs in Silk-Collagen

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Human BM-MSCs (ATCC, VA) were expanded following the manufacturer's instructions. Expanded BM-MSCs were trypsinized and resuspended in culture media (1 • 10 6 cells/mL) and mixed with the collagen control, 50%, and 100% silk-containing collagen pregels before 3D bioprinting (n = 3 for each concentration). Control BM-MSCs were cultured on standard plastic substrate. All experiments were conducted with cells at passages 2-4.

Sanz-Fraile H., Amoros S., Mendizabal I., Galvez-Monton C., Prat-Vidal C., Bayes-Genis A., Navajas D., Farre R, & Otero J. (2020). Silk-Reinforced Collagen Hydrogels with Raised Multiscale Stiffness for Mesenchymal Cells 3D Culture. Tissue engineering. Part A, 26(5-6).

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Profiling HCC Tissue-Adjacent Liver

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Ninety pairs of primary HCC tissue and adjacent non-tumor liver tissue (> 2 cm and < 5 cm from tumor edge) were collected from patients (56 males and 41 females; age, 41-73 years; mean age, 52.6 years) who underwent surgical resection at our hospital from October 2013 to January 2015. The tissue samples were immediately frozen and preserved at -80 0 C in liquid nitrogen. None of the patients suffered from hepatitis or had previously accepted anti-in ammatory treatment or oncotherapy.
Written informed consent was obtained from all patients before the study and the diagnosis of all tissue samples was made and independently con rmed by two pathologists. Overall survival was calculated during the duration between surgery and death or last follow-up. This study was approved by the Shenzhen Hospital, Peking University Institutional Ethics Committee and conducted following Ethical Principles for Medical Research Involving Human Subjects of the Helsinki Declaration.
Cell culture HCC cell lines (HL7702, HCCLM3, HepG2, and MHCC97-H) and human immortalized hepatocyte line L02, and human BM-MSCs were obtained from the American Type Culture Collection. The cells were all cultured with DMEM containing 10% fetal bovine serum and 1% penicillin-streptomycin.

Lin Z., Ren Q., Ma X., & Jikui L. (2021). Bone Marrow Mesenchymal Stem Cells-Derived Exosomes Upregulate MicroRNA-424-5p to Suppress Hepatocellular Carcinoma Cell Growth by Inhibiting Forkhead Box K1.

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Culturing Human Bone Marrow Mesenchymal Stem Cells

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Human BM-MSCs (American Type Culture Collection, Manassas, VA, USA) were cultured in 175 cm² cell culture flasks (Corning, Tewksbury, MA, USA) in modified Eagle's medium alpha supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (GIBCO, Carlsbad, CA, USA) at 37°C in a humidified 5% CO₂ atmosphere. Upon reaching 70% confluence, cells were dissociated by 0.25% trypsin-EDTA (Invitrogen Carlsbad, CA, USA) and reseeded into 96-well and 12-well plates for the next stage of the experiments.

Chen X., He F., Zhong D.Y, & Luo Z.P. (2015). Acoustic-Frequency Vibratory Stimulation Regulates the Balance between Osteogenesis and Adipogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells. BioMed Research International, 2015, 540731.

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