Mn 640 w filter paper

Elemental content of shoots and roots was measured after the acidic digestion of the plant samples. Dried (2 days at 85 °C) plant samples were subjected to digestion in ccH₂O₂ for 1 h, then ccHNO₃ for about 15 min at 60 °C and 45 min at 120 °C. The solution was thoroughly homogenized, and eventually filtered through MN 640 W filter paper (Macherey-Nagel, Düren, Germany). ICP-OES (inductively coupled plasma–optical emission spectrometer, Spectro Genesis, SPECTRO, Freital, Germany) was used for determining elemental content of the filtrate. A multielement standard for 33 elements was used for calibration (Loba Chemie Product code: I166N, Loba Chemie PVT, Mumbai, India).

Element concentration estimation was performed after acid digestion of dried plant samples. The samples were dried for two days at 85 °C and then digested in ccH₂O₂ for 1 h, followed by the addition of ccHNO₃, and incubation for 15 min at 60 °C and 45 min at 120 °C. The resulting solution was filtered through MN 640 W filter paper (Macherey-Nagel, Düren, Germany) and thoroughly homogenized. The filtrate was then analyzed for elemental content using an inductively coupled plasma–optical emission spectrometer (ICP-OES) (Spectro Genesis, SPECTRO, Freital, Germany) with the help of a multi-element standard for 33 elements (Loba Chemie Product code: I166N, Loba Chemie PVT, Mumbai, India) used for calibration.