Erk 1 2 sc 135900

Heat-inactivated fetal bovine serum (FBS), trypsin/EDTA and Dulbecco’s modified Eagle’s medium (DMEM) containing penicillin (100 UI/mL) and streptomycin (100 |Jg/mL) were from Gibco (Grand Island, NY) or Cultilab (Campinas, Brazil). Earle’s Minimal Essential Medium (EMEM) was purchased from Invitrogen (Carlsbad, CA). Vybrant DiO and DiI Cell-Labeling Solution were purchased from Thermo Scientific (Waltham, MA). Antibodies for p-Akt (sc-7985-R), p-Erk (sc-7383) and Erk 1/2 (sc - 135900) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies for Akt (#9272), p21 (2946S), PARP (#9542), cleaved caspase-3 (#9661) and p-JNK (#9255S) were purchased from Cell Signaling Technology (Beverly, MA). Rat monoclonal anti-Gal-3 antibody was obtained from the hybridoma cell line TIB-166 of the American Type Culture Collection (ATCC, Manassas, VA). Water was collected from a Milli-Q purification system from EMD Millipore (Bedford, USA). Unless stated otherwise, remaining reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, USA).