The protein expressions of β-actin, VEGF, bFGF, Ang1, Ang2, PDGF-B, PDGF-D, and PlGF were studied using Western blotting. The primary antibodies used in this study were anti-β-actin (4970, Cell Signaling Technology, Danvers, MA, USA), anti-VEGF (sc-152, Santa Cruz Biotechnology, Dallas, TX, USA), anti-bFGF (sc-79, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Ang1 (ab95230, Abcam, Cambridge, MA, USA), anti-Ang2 (ab56301, Abcam), anti-PDGF-B (ab23914, Abcam), anti-PDGF-D (40–2100, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), and anti-PlGF (sc-1883, Santa Cruz Biotechnology). After treatment with different hcy concentrations, the eyes were collected from the animals. A lysis buffer was used to extract retinal/choroidal proteins. The protein concentration was standardized, and the proteins were detected and quantified via electrochemiluminescence (Plus-ECL, PerkinElmer, Waltham, MA, USA) and densitometry.
Anti vegf sc 152
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-VEGF (sc-152) is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that specifically binds to and neutralizes the Vascular Endothelial Growth Factor (VEGF) protein. This product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Ecl plus, by PerkinElmer (1 mentions)
Ab56301, by Abcam (1 mentions)
Ab23914, by Abcam (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Ab95230, by Abcam (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Biotinylated isolectin b4, by Vector Laboratories (1 mentions)
Alexa fluor 488 conjugated anti gfp a21311, by Thermo Fisher Scientific (1 mentions)
Cy3 cy5 dylight549 dylight649 conjugated iggs, by Jackson ImmunoResearch (1 mentions)
Fluorescent streptavidin conjugates, by Thermo Fisher Scientific (1 mentions)
Hsc70 sc 7298, by Santa Cruz Biotechnology (1 mentions)
4 protocols using anti vegf sc 152
1
Protein Expression Profiling in Retinal Angiogenesis
2
Immunohistochemical Analysis of Angiogenesis Markers
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A heat‐induced antigen retrieval was applied using a microwave treatment (two times, 5 min., 500 W in 10 mM citrate pH 6.0) before sections were incubated overnight at room temperature in a humid chamber with the following primary polyclonal antisera, all raised in rabbit: (i) anti‐VEGF (sc‐152, dilution 1:50; Santa Cruz Biotechnologies, Santa Cruz, CA, USA); (ii) anti‐ve‐cadherin (VE‐CAD, abcam, ac33168, dilution 1:200; Abcam, Cambridge, UK); (iii) anti‐ENDO (ENDO, code 2161183, dilution 1:200; Millipore, Bedford, MA, USA).
3
Multicolor Immunohistochemistry of Tissues
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Immunohistochemistry of whole-mount samples or tissue sections was performed as previously described (Kubota et al., 2009 (link)). The primary monoclonal antibodies used were hamster anti-CD31 (MAB1398Z; EMD Millipore), PDGFRα (14-1401; eBioscience), vascular endothelial cadherin (550548; BD), and F4/80 (MCA497R; Serotec). The primary polyclonal antibodies used were Alexa Fluor 488–conjugated anti-GFP (A21311; Molecular Probes), collagen IV (LSL-LB-1407; Cosmo Bio), anti-VEGF (sc-152; Santa Cruz Biotechnology, Inc.), and cleaved caspase-3 (9664; Cell Signaling Technology). The secondary antibodies used were Alexa Fluor 488 fluorescence–conjugated IgGs (Molecular Probes) or Cy3/Cy5 DyLight 549/DyLight 649–conjugated IgGs (Jackson ImmunoResearch Laboratories, Inc.). For nuclear staining, specimens were treated with DAPI (Molecular Probes). In some experiments, blood vessels and monocyte lineage cells were simultaneously visualized using biotinylated isolectin B4 (B-1205; Vector Laboratories) followed by fluorescent streptavidin conjugates (Molecular Probes).
4
Western Blot Analysis of VEGF
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Western blot analysis was performed as described previously (Okabe et al., 2014 (link)). The primary antibodies used were anti-VEGF (sc-152; Santa Cruz Biotechnology, Inc.) and HSC 70 (sc-7298; Santa Cruz Biotechnology, Inc.). The fluids of the vitreous cavity were harvested by rinsing posterior segments of eyeballs with PBS and then thoroughly removing the few cellular components via centrifugation.
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