Total RNAs were extracted from differently treated cells by TRIzol reagent (Invitrogen, USA) according to the instructions of manufacturer. After the quantitative analyses using spectrophotometer, 2–5 µg RNAs were reverse transcribed into cDNAs by RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, USA). Thereafter, cDNAs were analyzed through the iQ5 qRT‐PCR instrument (Bio‐Rad). The primer sequences for this study are listed in Table S1 (Supporting Information).
Iq5 qrt pcr instrument
Manufactured by Bio-Rad
The IQ5 qRT-PCR instrument is a real-time quantitative reverse transcription PCR (qRT-PCR) system designed for gene expression analysis. It provides accurate and reliable data for measuring the expression levels of target genes. The IQ5 instrument is capable of performing qRT-PCR experiments and collecting data in real-time.
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Lab products found in correlation
2 protocols using iq5 qrt pcr instrument
1
RNA Extraction and qRT-PCR Analysis
2
Quantitative Real-Time PCR Analysis
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Total RNAs were extracted from cells with Trizol reagent (Invitrogen, USA), and then 2–5 μg RNAs were reverse-transcribed into cDNA with M-MLV reverse transcriptase. qPCR was performed on an iQ5 qRT-PCR instrument (Bio-Rad), as described previously78 (link), 79 (link) GAPDH was used as a loading control for normalization. The primer sequences are listed in Supplementary Table 1 .
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