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Agilent 1050

Manufactured by Agilent Technologies
Sourced in United States

The Agilent 1050 is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a compact modular design, advanced electronics, and precise flow control for reliable and reproducible results.

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2 protocols using agilent 1050

1

HPLC and SFE-CO2 for Motion Sickness

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The high-performance liquid chromatography (HPLC) system used consisted of a low-pressure quaternary pump (model Agilent 1050), an autosampler (model Agilent 1050), and a ultraviolet (UV) visible detector (G79853A), and it employed an Agilent ZORBAX Eclipse DB C18 column (250 mm × 4.6 mm, 5 μm) (Agilent 1050 ChemStation, USA). The SFE-CO2 experiments were performed using a CL-10-J-3 SFE-CO2 device (He Si Corp., Beijing, China). Motion sickness was simulated using a DSL-1 minitype animal centrifuge unit (Peace Medical Equipment Factory, Beijing, China).
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2

Analytical Techniques for Biomolecule Characterization

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UV-Vis analysis was performed using a USB-2000 fiber optic spectrometer (Ocean Optics, Inc.) with a DT-Mini-B lamp source. HPLC analysis was performed using modular Hewlett Packard Agilent 1050 and 1260 series instruments with an attached vacuum degasser, autosampler and diode array detector. C18 Hypersil (5 μm, 150 × 3.2 mm, Varian) columns were used as stated in the methods below. HPLC-MS was performed using a modular Agilent 1100 series with DAD coupled with a Q-Trap mass spectrometer (ABI) in a positive ion mode. For HPLC-MS analysis C18 Hypersil (5 μm, 150 × 3.2 mm, Varian) column was used. ESI-MS analysis was performed using a 3200 Q-Trap mass spectrometer in infusion mode.
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