CTX, striatum, and liver were dissected as previously reported [13 (link)–15 ] and the wet weight of each sample was obtained. Tissue was transferred to a Teflon digestion vessel and 2 ml of 50% HNO3 was added. Samples were digested in the MARS Xpress system (CEM) for 15 min at 200°C and then diluted with H2O to bring the final acid concentration to 10% as described [16 (link)]. For introduction into the instrument, samples were diluted to 12.5–200 μl of digested tissue sample per ml with 2% HNO3. Total iron content of each sample was determined by analysis with the X Series ICP-MS (Thermo Fisher Scientific) in Collision Cell Technology (CCT) mode using H/He as the gas. A standard curve for Fe was prepared from 0–100 ppb and internal standards Ga (50 ppb) and Y (100 ppb) were used. The iron content was normalized to wet weight and the results were reported as nmol Fe/g tissue (wet weight). Statistical analysis was done using GraphPad Prism.
X series icp ms
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The X Series ICP-MS is a powerful inductively coupled plasma mass spectrometry (ICP-MS) instrument designed for elemental analysis. It provides multi-element detection capabilities with high sensitivity and accuracy. The instrument utilizes an inductively coupled plasma to ionize and introduce samples into a mass spectrometer, which then separates and detects the various elements present in the sample.
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4 protocols using x series icp ms
1
Quantitative Iron Content Analysis
2
Elemental Composition Analysis of Clay Leachates
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For clay samples in solution (i.e., leachates, 1 mg/mL), ICP-MS was employed to determine the inorganic elemental composition. The leachates were acidified to 10% nitric acid and processed directly through the ICP-MS. The ICP was calibrated and checked using a certified geochemical reference material, Japanese basalt JB-3a (Fuji volcano; [34 ]). The leachates were analyzed using a Thermo-Elemental X-Series ICP-MS equipped with a Cetac AS-500 Auto-sampler (ThermoFisher Scientific, Newport, UK). Elements present in the solution were analyzed on mass-to-charge ratios and compared to a geological standard. This was repeated three times per leachate and the mean values and standard deviations were used to determine the results. Raw data was corrected for blanks, controls, and dilutions.
3
Characterization of Nanomaterial Morphology and Composition
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The surface morphologies of samples were characterized by scanning electron microscopy (SEM, JSM-6300, JEOL Ltd, Japan) and transmission electron microscope JEM-2100 (JEOL Ltd, Japan). XRD patterns were recorded using a Bruker D8 Advance X-ray diffractometer to determine the crystalline phase of the nanomaterials. Ultrasonic cleaner (JY92-IIN) was purchased from Ningbo Xinzhi Biotechnology Co., Ltd. The concentration of Pb(ii ) in tobacco leaves sample was determined by ICP-MS (Thermo, X-series ICP-MS). Fourier Transform Infrared (FTIR, Nicolet iS5, USA) was used to analyze functional group status of the prepared materials. All electrochemical experiments were carried out on CHI660E electrochemical workstation (ChenHua Instruments Co., Shanghai, China). The bare glassy carbon electrode (GCE, diameter 3 mm) or modified GCE was the working electrode; the platinum electrode was used as the counter electrode, and the saturated Ag/AgCl electrode was used as the reference electrode to complete the standard three-electrode system.
4
Nitric Acid Treatment for ICP-MS
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