Prism 8.4.0 for windows

Western blot analyses were essentially performed as described previously [48 (link), 56 (link)] with 30 µg of protein loaded from each sample on Tris–glycine or Bis–Tris SDS polyacrylamide gels and separated electrophoretically. Afterwards, proteins were transferred to 0.2 µm nitrocellulose membranes (GE Healthcare, Dornstadt, Germany) using the respective transfer buffer. After blocking, the membranes were incubated with the same primary antibodies as used in the RPMA analysis (for details and dilutions see Additional file 1: Table S2) diluted in TBST at 4 °C overnight. Following the incubation with a fluorescence- or HRP-labeled secondary antibody, membranes were detected using an Odyssey Fc Imager (LI-COR Biosciences, Bad Homburg, Germany) and quantified using the Image Studio Software (LI-COR) or ImageJ. Statistical analyses were performed using GraphPad Prism 8.40 for Windows (GraphPad Software, San Diego, CA). Data is shown as arithmetic means ± SEM. Outliers were identified using Grubbs’ test with alpha = 0.05. Statistical significance of data sets was determined using Student’s t-test and p-values less than or equal to 0.05 were considered as statistically significant.