Cell viability was measured using the CellTiter-Glo luminescent cell viability assay (Promega, Madison, WI, USA), following the manufacturer’s instructions. Approximately 3 × 103 cells were transferred to white 96 well plates. The next day, the culture medium was removed, and the desired concentrations of AUY922 or/and lapatinib (CP358774) were added to a volume of 100 μl. After 72 h, 100 μl of CellTiter-Glo reagent was added, and the plates were incubated for 10 min at room temperature. The luminescence was measured using a Wallac 1420 apparatus (PerkinElmer, Boston, MA, USA).
Wallac 1420 apparatus
Manufactured by PerkinElmer
Sourced in United States
The Wallac 1420 apparatus is a versatile laboratory instrument designed for various applications. It features a modular design with multiple detection modes, including luminescence, fluorescence, and absorbance. The Wallac 1420 provides researchers with a flexible and reliable platform to perform a wide range of analytical and assay-based experiments.
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Lab products found in correlation
2 protocols using wallac 1420 apparatus
1
Cell Viability Assay with AUY922 and Lapatinib
2
Caspase 3/7 Activation Luminescence Assay
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Caspase 3 and 7 activations were measured using the Caspase-Glo 3/7 Luminescence Assay (Promega Corp., Madison, WI, USA), following the manufacturer’s protocol. Protein samples from cells were prepared using RIPA buffer in the same manner as Western blot sample preparation. Ten micrograms of protein samples in 100 μl total volume were transferred to white 96 wells, and 100 μl of equilibrated Caspase-Glo 3/7 reagent was added to protein samples and incubated for 1 h at room temperature. Luminescence was measured using the Wallac 1420 apparatus (PerkinElmer, Waltham, MA, USA).
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