Pe conjugated mouse anti human gpa moab

Manufactured by Agilent Technologies

Sourced in Germany, United States

The PE-conjugated mouse anti-human GPA MoAb is a laboratory tool used for the detection and analysis of glycophorin A (GPA) expression on human cells. It is a monoclonal antibody conjugated with the fluorescent dye phycoerythrin (PE), allowing for the identification and quantification of GPA-positive cells by flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Facscanto 2, by BD (3 mentions) Pe conjugated mouse anti human cd42b moab, by Agilent Technologies (3 mentions) Axion software 4, by Zeiss (2 mentions) Fitc conjugated mouse anti human cd41 moab, by Agilent Technologies (2 mentions) Axiocam erc5s digital camera, by Zeiss (2 mentions) Ax10scopea1 microscope, by Zeiss (2 mentions)

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Anti-TM (1 citations)

3 protocols using pe conjugated mouse anti human gpa moab

Differentiation of CD34+ Cells

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Differentiation of CD34+ cells was assessed by morphological analysis of May-Grunwald-Giemsa-(MGG)-stained cytospins at 4, 8 and 12 days post-nucleofection and by flow-cytometric analysis of differentiation markers expression (CD34, CD38, CD71, CD36, Glycophorin A (GPA), myeloperoxydase (MPO), CD163, CD41 and CD42b) at 4, 8 and 12 days post-nucleofection.
Micrographs of the MGG-stained cytospins were taken by means of anAxioscopeA1 microscope provided with AxioCam ERc 5S Digital Camera (Carl Zeiss MicroImaging Inc.; Thornwood, NY, USA). The following monoclonal antibodies (MoAbs) were used for flow cytometric analysis: fluorescein isothiocyanate (FITC)-conjugated mouse anti-CD34 MoAb, phycoerythrin (PE)-conjugated mouse anti-human CD38 MoAb, FITC-conjugated mouse anti-human CD36 MoAb, allophycocyanin (APC)-conjugated mouse anti-human CD71 MoAb, APC-conjugated mouse anti-human CD163 MoAb (all from Miltenyi Biotec, Bergisch Gladbach, Germany), FITC-conjugated mouse anti-CD41 MoAb, PE-conjugated mouse anti-human CD42b MoAb, PE-conjugated mouse anti-human GPA MoAb (all from Dako; Milano, Italia; http://www.dako.com) and FITC-conjugated mouse anti-human MPO MoAb (from BD Biosciences; San Jose, CA, USA). Flow cytometric analyses were performed by means of a BD FACSCanto II (BD Biosciences; San Jose, CA, USA). At least 10,000 events for sample were acquired.

Bianchi E., Ruberti S., Rontauroli S., Guglielmelli P., Salati S., Rossi C., Zini R., Tagliafico E., Vannucchi A.M, & Manfredini R. (2017). Role of miR-34a-5p in Hematopoietic Progenitor Cells Proliferation and Fate Decision: Novel Insights into the Pathogenesis of Primary Myelofibrosis. International Journal of Molecular Sciences, 18(1), 145.

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Multilineage Hematopoietic Differentiation

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Grunwald-Giemsa-stained cytospins and by flow cytometry analysis of CD34, CD14, CD66b, CD15, Glycophorin A (GPA), CD41 and CD42b surface antigen expression at day 5, 7, 10, and 12 after the last nucleofection. Images were captured by using an Ax10scopeA1 microscope equipped with AxioCam ERc 5S Digital Camera and Axion software 4.8 (all Carl Zeiss MicroImaging Inc.; Thornwood, NY, USA). The images were then processed with Adobe Photoshop 11.0.2 software.
The following monoclonal antibodies (MoAbs) were used for flow cytometry analysis: phycoerythrin (PE)-conjugated mouse anti-human CD14 MoAb, fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD34 MoAb, FITC-conjugated mouse anti-human CD66b MoAb, FITC-conjugated mouse anti-human CD15 MoAb (all from Miltenyi Biotech; Auburn, CA, USA), FITC-conjugated mouse anti-human CD41 MoAb, PE-conjugated mouse anti-human CD42b MoAb, and PE-conjugated mouse anti-human GPA MoAb (all from Dako; Milano, Italia). After staining, cells were analyzed by using a BD FACSCanto II (BD Biosciences; San Jose, CA USA). At least 10,000 events were counted for each sample to ensure statistical relevance.

Zini R., Rossi C., Norfo R., Pennucci V., Barbieri G., Ruberti S., Rontauroli S., Salati S., Bianchi E, & Manfredini R. (2016). miR-382-5p Controls Hematopoietic Stem Cell Differentiation Through the Downregulation of MXD1. Stem cells and development, 25(19).

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Characterization of CD34+ Cell Differentiation

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Differentiation of CD34 + cells was monitored by morphological analysis of May-Grunwald-Giemsa-stained cytospins and by flow cytometric analysis of differentiation markers' expression (Glycophorin A [GPA], CD41, and CD42b) on day 9, 12, and 14 after the last nucleofection or retroviral infection. Images were captured by using an Ax10scopeA1 microscope equipped with AxioCam ERc 5S Digital Camera and Axion software 4.8 (all Carl Zeiss Mi-croImaging, Inc., Thornwood, NY). The images were then processed with Adobe Photoshop 7.0 software. The following monoclonal antibodies (MoAbs) were used for flow cytometric analysis: FITC-conjugated mouse antihuman CD41 MoAb, PE-conjugated mouse antihuman CD42b MoAb, and PE-conjugated mouse anti-human GPA MoAb (all from Dako; Milano, Italia; www.dako.com). After staining, cells were analyzed by using a BD FACSCanto II (BD Biosciences, San Jose, CA). At least 10,000 events were counted for each sample to ensure statistical relevance.

Salati S., Prudente Z., Genovese E., Pennucci V., Rontauroli S., Bartalucci N., Mannarelli C., Ruberti S., Zini R., Rossi C., Bianchi E., Guglielmelli P., Tagliafico E., Vannucchi A.M, & Manfredini R. (2018). Calreticulin Affects Hematopoietic Stem/Progenitor Cell Fate by Impacting Erythroid and Megakaryocytic Differentiation. Stem cells and development, 27(4).

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