THP1, U937, and NB4 cells were cultured in RMPI 1640 (Thermo Fisher) with 10% fetal bovine serum (FBS). KG1 cells were cultured in Iscove modified Dulbecco medium (IMDM) with 20% FBS, and K562 cells were cultured in IMDM with 10% FBS. Samples of patient with primary AML were cultured in IMDM, 15% bovine serum albumin, insulin, and transferrin (StemCell Technologies), 100 ng/mL stem cell factor (SCF), 50 ng/mL FMS-like tyrosine kinase 3 ligand, 20 ng/mL interleukin-3, 20 ng/mL granulocyte colony-stimulating factor (PeproTech) 10−4 M β-mercaptoethanol, 500 nM SR1 (Stemcell Technologies), and 500 nM UM729 (Stemcell Technologies). Mobilized peripheral blood cells were cultured in StemSpan-ACF (Stemcell Technologies) containing SCF at 100 ng/mL, thrombopoietin at 50 ng/mL, FLT3-L at 100 ng/mL, Glutamax 1×, low density lipoprotein at 10 μg/mL, and 35 nM UM171.
Fms like tyrosine kinase 3
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
FMS-like tyrosine kinase 3 is a receptor tyrosine kinase that plays a role in the regulation of hematopoietic stem cell development. It is involved in cell signaling pathways and is expressed in various cell types, including hematopoietic progenitor cells.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by STEMCELL (1 mentions)
Transferrin, by STEMCELL (1 mentions)
Granulocyte colony stimulating factor, by Thermo Fisher Scientific (1 mentions)
Um171, by STEMCELL (1 mentions)
Rmpi 1640, by Thermo Fisher Scientific (1 mentions)
Stemspan acf, by STEMCELL (1 mentions)
β mercaptoethanol, by STEMCELL (1 mentions)
Um729, by STEMCELL (1 mentions)
Stem cell factor (scf), by Thermo Fisher Scientific (1 mentions)
Serum free cell culture medium, by Lonza (1 mentions)
2 protocols using fms like tyrosine kinase 3
1
Optimized Culture Conditions for Diverse Hematopoietic Cell Lines
2
Hypoxic Expansion of UCB-MNCs
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Isolated UCB-MNC were cultured under 0.5% O2, for 18 h, at a density of 2 million cells/mL in serum-free cell culture medium (Lonza AG, Basel, Switzerland) supplemented with 0.5 μg/mL FMS-like tyrosine kinase-3 (Peprotech, London, UK) and 0.5 μg/mL stem-cell factor (Peprotech, London, UK).
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